Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,354,944	C→T	A227V (GCC→GTC)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,354,944	0	C	T	100.0%	88.4 / NA	25	A227V (GCC→GTC)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

GCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCG  >  NC_000913/2354867‑2355021                                                                              |                                                                              gCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGa                                                                            <  1:371225/81‑1 (MQ=255)         tACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAAc                                                                    >  1:371250/1‑81 (MQ=255)             tGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACtgtg                                                                >  2:371262/1‑81 (MQ=255)               aTGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGc                                                              <  1:371226/81‑1 (MQ=255)               aTGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGc                                                              >  1:371256/1‑81 (MQ=255)                  cgcTTATTCCTGTCGCCAATACCTGCGATATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgtt                                                           <  1:371227/81‑1 (MQ=255)                    cTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgttgg                                                         <  1:371228/81‑1 (MQ=255)                     ttATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgttggt                                                        >  2:371253/1‑81 (MQ=255)                       aTTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgttggt                                                        <  2:371229/79‑1 (MQ=255)                        ttCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgttggttg                                                      <  1:371230/80‑1 (MQ=255)                         tCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTtgaa                                                    >  2:371267/1‑81 (MQ=255)                            tGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTtgaatg                                                  >  1:371260/1‑80 (MQ=255)                              tCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTtgaatgaa                                                <  2:371231/80‑1 (MQ=255)                                gCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGaaaa                                              <  2:371233/80‑1 (MQ=255)                                gCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGaaaa                                              <  2:371232/80‑1 (MQ=255)                                    aTACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTAc                                         >  1:371265/1‑81 (MQ=255)                                            gAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCa                                 >  1:371257/1‑81 (MQ=255)                                            gAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCa                                 >  2:371255/1‑81 (MQ=255)                                                 gATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGAt                            >  1:371252/1‑81 (MQ=255)                                                   tCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGc                          <  1:371234/81‑1 (MQ=255)                                                      tGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCttt                       >  1:371268/1‑81 (MQ=255)                                                              gCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACg               <  2:371235/81‑1 (MQ=255)                                                                   cTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTgcc          <  1:371236/81‑1 (MQ=255)                                                                      cGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTgccgcc       >  2:371254/1‑81 (MQ=255)                                                                           tGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCg  >  2:371277/1‑81 (MQ=255)                                                                              |                                                                              GCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCG  >  NC_000913/2354867‑2355021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑