Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,355,061	T→C	F266S (TTT→TCT)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,355,061	0	T	C	100.0%	70.3 / NA	21	F266S (TTT→TCT)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/6);  total (15/6)

TGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTG  >  NC_000913/2354992‑2355131                                                                      |                                                                       tGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAgg                                                             >  1:371283/1‑81 (MQ=255)     gCTTTTGCCAACGCTGCCGCCTTACGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTgg                                                         >  1:371273/1‑81 (MQ=255)      cTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGc                                                        >  1:371276/1‑81 (MQ=255)           gCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCgtgt                                                    <  1:371243/80‑1 (MQ=255)           gCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGtgtg                                                   >  2:371275/1‑81 (MQ=255)                  cTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCg                                            >  1:371270/1‑81 (MQ=255)                     ccgccTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGc                                         <  2:371244/81‑1 (MQ=255)                       gccTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGa                                       >  2:371280/1‑81 (MQ=255)                        ccTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGAt                                      <  2:371245/81‑1 (MQ=255)                             cGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGt                                 <  2:371246/81‑1 (MQ=255)                               tGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGa                               <  2:371247/81‑1 (MQ=255)                                 cTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGaaa                             <  1:371248/81‑1 (MQ=255)                                   gggCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGaaaa                            >  1:371278/1‑80 (MQ=255)                                   gggCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGaaaa                            >  2:371281/1‑80 (MQ=255)                                       aTTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGt                        >  2:371274/1‑80 (MQ=255)                                       aTTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGt                        >  2:371290/1‑80 (MQ=255)                                             cTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTg                  >  1:371287/1‑80 (MQ=255)                                             cTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTg                  >  2:371272/1‑80 (MQ=255)                                                cAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTaa               >  1:371279/1‑80 (MQ=255)                                                     ccAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATgg         >  2:371282/1‑81 (MQ=255)                                                             aGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTg  >  1:371285/1‑80 (MQ=255)                                                                      |                                                                       TGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTG  >  NC_000913/2354992‑2355131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑