Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,359,162	C→T	A331T (GCG→ACG)	yfaV ←	putative transporter YfaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,359,162	0	C	T	100.0%	118.6 / NA	33	A331T (GCG→ACG)	yfaV	putative transporter YfaV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

AGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCC  >  NC_000913/2359086‑2359239                                                                             |                                                                              aGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCAtt                                                                           <  1:371913/81‑1 (MQ=255)    tgtgTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACACCGCTACGATCGTCATTACt                                                                        >  1:371946/1‑81 (MQ=255)     gtgtCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTg                                                                       >  2:371970/1‑81 (MQ=255)       gtCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTg                                                                       >  1:371966/1‑79 (MQ=255)        tCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGga                                                                     <  1:371914/80‑1 (MQ=255)                gTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAagcagc                                                            <  2:371915/81‑1 (MQ=255)                 tCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGcc                                                           <  1:371916/81‑1 (MQ=255)                   cAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGccc                                                          <  2:371917/80‑1 (MQ=255)                    aGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCg                                                         >  1:371958/1‑80 (MQ=255)                    aGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCg                                                         >  1:371971/1‑80 (MQ=255)                      aaCACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGa                                                       >  1:371975/1‑80 (MQ=255)                      aaCACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGAc                                                      <  1:371918/81‑1 (MQ=255)                            ggCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGCTCGTCATTACTGGAGAAAGCAGCCCGGACAGacc                                                 >  1:371959/1‑80 (MQ=255)                            ggCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGacc                                                 >  1:371962/1‑80 (MQ=255)                             gCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGacc                                                 <  2:371919/79‑1 (MQ=255)                                    ggCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                        <  1:371921/81‑1 (MQ=255)                                    ggCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                        <  1:371920/81‑1 (MQ=255)                                     gCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATg                                       <  2:371922/81‑1 (MQ=255)                                       aaTAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                     >  2:371965/1‑81 (MQ=255)                                         tAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccg                                   <  1:371923/81‑1 (MQ=255)                                          aaaCCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgc                                  >  2:371979/1‑81 (MQ=255)                                                     cTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATg                       >  2:371967/1‑81 (MQ=255)                                                        cAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                      <  2:371924/79‑1 (MQ=255)                                                         aaCACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCTCCGCCAGTAATGTCAg                   >  2:371964/1‑81 (MQ=255)                                                         aaCACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                   <  2:371925/81‑1 (MQ=255)                                                            acacaGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAgcgc                >  2:371957/1‑81 (MQ=255)                                                              acaGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTg              >  1:371963/1‑81 (MQ=255)                                                                    cTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACAt        <  2:371926/81‑1 (MQ=255)                                                                       cGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACAtt       <  2:371927/79‑1 (MQ=255)                                                                       cGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACAtt       <  2:371929/79‑1 (MQ=255)                                                                       cGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTAc     <  2:371928/81‑1 (MQ=255)                                                                         aTCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGc   >  1:371974/1‑81 (MQ=255)                                                                          tCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGcc  <  2:371930/81‑1 (MQ=255)                                                                             |                                                                              AGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCC  >  NC_000913/2359086‑2359239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑