Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,359,509	A→G	L215P (CTT→CCT)	yfaV ←	putative transporter YfaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,359,509	0	A	G	100.0%	107.4 / NA	31	L215P (CTT→CCT)	yfaV	putative transporter YfaV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/17);  total (14/17)

CAGACTCGGCCATTACGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAAGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCAAAAGAATGTGAATACC  >  NC_000913/2359431‑2359583                                                                               |                                                                           cAGACTCGGCCATTACGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGc                                                                          <  2:371995/81‑1 (MQ=255)         gCCATTACGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGtttt                                                                  <  2:371996/81‑1 (MQ=255)           cATTACGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGtttttt                                                                >  2:372025/1‑81 (MQ=255)           cATTACGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGtttttt                                                                >  2:372033/1‑81 (MQ=255)            aTTACGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTc                                                               <  1:371997/81‑1 (MQ=255)              tACGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCtt                                                             >  2:372035/1‑81 (MQ=255)                cGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGt                                                           <  1:371998/81‑1 (MQ=255)                   aGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTa                                                        >  2:372027/1‑81 (MQ=255)                     cgcATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACt                                                      <  1:371999/81‑1 (MQ=255)                      gcATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTc                                                     <  1:372000/81‑1 (MQ=255)                           gCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGaaa                                                <  1:372001/81‑1 (MQ=255)                               aGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAAc                                              <  1:372002/79‑1 (MQ=255)                               aGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACg                                             <  2:372003/80‑1 (MQ=255)                               aGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGt                                            >  1:372042/1‑81 (MQ=255)                                 ccGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGc                                          >  1:372069/1‑81 (MQ=255)                                   gagaAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCt                                        >  1:372039/1‑81 (MQ=255)                                   gagaAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCt                                        <  2:372004/81‑1 (MQ=255)                                         tCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCg                                  <  1:372005/81‑1 (MQ=255)                                           aCTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCgg                                 <  1:372006/80‑1 (MQ=255)                                           aCTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGgt                                >  1:372034/1‑81 (MQ=255)                                            cTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGgtg                               <  2:372007/81‑1 (MQ=255)                                               tCTGTTGTTCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGgtgtgt                            >  1:372030/1‑81 (MQ=255)                                                   ttgttCTTCACTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGtc                           <  1:372008/78‑1 (MQ=255)                                                            aCTTGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCaaaa               <  1:372009/81‑1 (MQ=255)                                                               tGCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCAAAAGaa            >  2:372052/1‑81 (MQ=255)                                                                gCCAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCAAAAGa             <  1:372010/79‑1 (MQ=255)                                                                  cAGTTGATTGATAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCAAAAGAAtgt         >  2:372043/1‑81 (MQ=255)                                                                     ttgattgaTAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCCCCGGTGTGTCATCAAGCCAAAAGAATGTGaa      >  2:372032/1‑81 (MQ=255)                                                                      tgattgatAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCAAAAGAATGTGaa      >  2:372044/1‑80 (MQ=255)                                                                        attgatAGGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCAAAAGAATGTGAATAc   >  1:372056/1‑81 (MQ=255)                                                                         ttgatAGGCAACGTTTTTTCTTGTTTACTCAAAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCAAAAGAATGTGAATAcc  <  2:372011/81‑1 (MQ=255)                                                                               |                                                                           CAGACTCGGCCATTACGCAGCGCATCGCTCAGCCGAGAAGTCACTTTCTGTTGTTCTTCACTTGCCAGTTGATTGATAAGCAACGTTTTTTCTTGTTTACTCAGAAAACGTGCCTGCTCCGGTGTGTCATCAAGCCAAAAGAATGTGAATACC  >  NC_000913/2359431‑2359583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑