Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,398,385	T→A	D594V (GAT→GTT)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,398,385	0	T	A	100.0%	106.7 / NA	30	D594V (GAT→GTT)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/11);  total (19/11)

GTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTT  >  NC_000913/2398310‑2398461                                                                            |                                                                             gtaccgtCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGaacaac                                                                          >  2:378521/1‑80 (MQ=255)  taccgtCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAacca                                                                        >  2:378518/1‑81 (MQ=255)   accgtCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAaccac                                                                       >  1:378540/1‑81 (MQ=255)      gtCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCAt                                                                    <  2:378484/81‑1 (MQ=255)         gCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCAc                                                                 >  1:378529/1‑81 (MQ=255)          cTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCAc                                                                 >  2:378522/1‑80 (MQ=255)                gCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGt                                                          >  1:378526/1‑81 (MQ=255)                           agcagAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAg                                               <  1:378485/81‑1 (MQ=255)                           agcagAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAg                                               >  2:378532/1‑81 (MQ=255)                             cagAAAGTACAAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAgcg                                             <  1:378486/81‑1 (MQ=255)                              agAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAgcg                                             >  2:378533/1‑80 (MQ=255)                                 aaGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCgcagc                                         <  2:378487/81‑1 (MQ=255)                                 aaGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCgcag                                          >  2:378547/1‑80 (MQ=255)                                   gTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCAt                                       <  1:378488/81‑1 (MQ=255)                                    tACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCAtt                                      <  1:378490/81‑1 (MQ=255)                                    tACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCAtt                                      <  1:378489/81‑1 (MQ=255)                                          aTGGGCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCAcgcg                                >  2:378552/1‑81 (MQ=255)                                              gCGTTTTCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATa                            <  2:378491/81‑1 (MQ=255)                                                   ttCCATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAg                        >  1:378544/1‑80 (MQ=255)                                                     ccATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAg                     >  1:378551/1‑81 (MQ=255)                                                      cATAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGc                    <  1:378492/81‑1 (MQ=255)                                                       aTAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGTCAGCGCAGCATTCACGCGGATAGCAGAAGCg                   >  2:378542/1‑81 (MQ=255)                                                       aTAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGTCAGCGCAGCATTCACGCGGATAGCAGAAGCg                   >  2:378546/1‑81 (MQ=255)                                                        tAATCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGt                  >  2:378560/1‑81 (MQ=255)                                                          aTCGCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGa                >  1:378545/1‑81 (MQ=255)                                                             gCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACg              <  2:378493/80‑1 (MQ=255)                                                             gCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGa             >  1:378537/1‑81 (MQ=255)                                                             gCTGTGCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGa             >  2:378539/1‑81 (MQ=255)                                                                gtgCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATg           >  2:378559/1‑80 (MQ=255)                                                                gtgCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATACACGCGGATAGCAGAAGCGTGACGATg           >  2:378549/1‑80 (MQ=255)                                                                 tgCGTTGATGAACAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATg           <  1:378494/79‑1 (MQ=255)                                                                        atgaaCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGtt  <  2:378495/81‑1 (MQ=255)                                                                            |                                                                             GTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTT  >  NC_000913/2398310‑2398461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑