Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,430,633	T→C	Y44C (TAC→TGC)	cvpA ←	colicin V production protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,430,633	0	T	C	100.0%	96.9 / NA	28	Y44C (TAC→TGC)	cvpA	colicin V production protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/19);  total (9/19)

CCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCA  >  NC_000913/2430562‑2430705                                                                        |                                                                         ccGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTgg                                                                 <  1:384086/81‑1 (MQ=255) ccGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTg                                                                  <  1:384087/80‑1 (MQ=255) ccGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTg                                                                  <  2:384085/80‑1 (MQ=255)   gcgATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTGAGTGTAGCAATGACTGGca                                                               <  1:384088/81‑1 (MQ=255)     gATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGcaac                                                             <  2:384089/81‑1 (MQ=255)      aTGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGcaaca                                                            <  1:384090/81‑1 (MQ=255)        ggCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGcaaca                                                            >  2:384135/1‑79 (MQ=255)          cAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACaaaga                                                        <  2:384091/81‑1 (MQ=255)           aaTCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACaaagaa                                                       <  2:384092/81‑1 (MQ=255)           aaTCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACaaaga                                                        >  1:384136/1‑80 (MQ=255)                cATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGca                                                   >  1:384126/1‑80 (MQ=255)                 aTTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGcac                                                  >  1:384139/1‑80 (MQ=255)                  tttCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACaa                                                <  2:384093/81‑1 (MQ=255)                  tttCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACaa                                                >  2:384130/1‑81 (MQ=255)                    tCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAAcc                                              >  1:384132/1‑81 (MQ=255)                     cGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAAcc                                              <  2:384094/80‑1 (MQ=255)                       aaCCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCa                                           <  1:384095/81‑1 (MQ=255)                                     cAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCg                                <  2:384096/78‑1 (MQ=255)                                      aaaGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATa                             <  2:384097/80‑1 (MQ=255)                                       aaGCCCGTAAACCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAAc                           >  2:384142/1‑81 (MQ=255)                                                aaCCAGACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACg                   <  2:384098/80‑1 (MQ=255)                                                     gACTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa             <  2:384099/81‑1 (MQ=255)                                                       cTGACAGGTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGc           <  2:384100/81‑1 (MQ=255)                                                             ggTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCGCGAACAAAGCCGCGGa     <  2:384101/81‑1 (MQ=255)                                                              gTAAGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt    >  2:384138/1‑81 (MQ=255)                                                              gTAAGTGTAGCAATGACTGGCAACAAAGAAAACACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt    <  2:384102/81‑1 (MQ=255)                                                                aaGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt    >  1:384143/1‑79 (MQ=255)                                                                 aGTGTAGCAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCa  <  2:384103/80‑1 (MQ=255)                                                                        |                                                                         CCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCA  >  NC_000913/2430562‑2430705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑