Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,439,254	T→C	E289E (GAA→GAG)	yfcJ ←	putative transporter YfcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,439,254	0	T	C	100.0%	91.6 / NA	27	E289E (GAA→GAG)	yfcJ	putative transporter YfcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/9);  total (18/9)

TCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCG  >  NC_000913/2439176‑2439325                                                                               |                                                                        tCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGtctc                                                                       >  2:385742/1‑81 (MQ=255) tCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGtctc                                                                       <  2:385715/81‑1 (MQ=255)  ccGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGtctc                                                                       >  2:385775/1‑80 (MQ=255)       tCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAg                                                                 >  1:385744/1‑81 (MQ=255)                 gccgcGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGAc                                                        >  1:385750/1‑80 (MQ=255)                              aTGCGACCCATGCACCTGGGGCTTGCCAGGGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACttt                                            <  2:385716/79‑1 (MQ=255)                                   aCCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCAcgccg                                     <  1:385717/81‑1 (MQ=255)                                    cccATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCAcgccgc                                    <  1:385718/81‑1 (MQ=255)                                     ccATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCAcgccgc                                    >  1:385755/1‑80 (MQ=255)                                     ccATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACgccgcc                                   <  2:385719/81‑1 (MQ=255)                                      cATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACgccgcc                                   >  1:385773/1‑80 (MQ=255)                                       aTGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCTCTTTCACGCCGCCa                                  >  2:385759/1‑80 (MQ=255)                                           aCCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACg                             >  1:385803/1‑81 (MQ=255)                                           aCCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACg                             >  2:385753/1‑81 (MQ=255)                                             cTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGt                           >  1:385767/1‑81 (MQ=255)                                                 ggCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCgg                       <  2:385720/81‑1 (MQ=255)                                                  gCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGc                      <  1:385721/81‑1 (MQ=255)                                                  gCTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGc                      <  2:385722/81‑1 (MQ=255)                                                   cTTGCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCa                     >  1:385770/1‑81 (MQ=255)                                                      gCCAGAGCAGCAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATc                   >  2:385756/1‑80 (MQ=255)                                                           agcagcAACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCa             <  1:385723/81‑1 (MQ=255)                                                               gcaACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACa         >  1:385762/1‑81 (MQ=255)                                                               gcaACAAGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACa         >  2:385766/1‑81 (MQ=255)                                                                caacaaGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACAt        >  1:385764/1‑81 (MQ=255)                                                                 aacaaGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACAt        >  2:385757/1‑80 (MQ=255)                                                                 aacaaGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACAt        >  1:385769/1‑80 (MQ=255)                                                                 aacaaGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATg       <  2:385724/81‑1 (MQ=255)                                                                    aaGCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGAcg    >  2:385761/1‑81 (MQ=255)                                                                      gCCCACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGAcgcg  >  1:385760/1‑81 (MQ=255)                                                                       cccACCGTCTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGAcgcg  >  2:385781/1‑80 (MQ=255)                                                                               |                                                                        TCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAACAAGCCCACCGTTTCTACAAGCAGAGAGACAATCGCCACTTTCACGCCGCCAAAACGGTCCGGCATCCAGCCAAACATGACGCG  >  NC_000913/2439176‑2439325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑