Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,451,016	A→G	D20D (GAT→GAC)	yfcQ ←	putative fimbrial protein YfcQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,451,016	0	A	G	100.0%	74.0 / NA	22	D20D (GAT→GAC)	yfcQ	putative fimbrial protein YfcQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/15);  total (7/15)

AATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCA  >  NC_000913/2450947‑2451088                                                                      |                                                                         aaTGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCTCGTGAAAGGTGATGTCTTCGTCAGCAGCAt                                                                >  2:387899/1‑80 (MQ=255)            caccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCACCAGCATGAGCTATGgcgc                                                    <  2:387862/81‑1 (MQ=255)                    aTGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGa                                              <  2:387863/79‑1 (MQ=255)                       cTACAGGTTGGCGGTGACAATAATGTTCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACaa                                         <  2:387864/81‑1 (MQ=255)                         aCAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAg                                       >  2:387891/1‑81 (MQ=255)                          cAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACaaa                                        <  1:387866/79‑1 (MQ=255)                          cAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACaaa                                        <  1:387865/79‑1 (MQ=255)                          cAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAg                                       <  1:387867/80‑1 (MQ=255)                             gTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAgg                                   <  1:387868/81‑1 (MQ=255)                              ttGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAggg                                  <  2:387869/81‑1 (MQ=255)                                 gCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGtt                                <  2:387870/80‑1 (MQ=255)                                 gCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGtt                                <  2:387871/80‑1 (MQ=255)                                             atGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCttt                    <  1:387873/80‑1 (MQ=255)                                             atGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTtc                   <  2:387872/81‑1 (MQ=255)                                                  ccGTGAAAGGTGATGTCTTCGTCAGCAGCATTAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCAtt              <  2:387874/81‑1 (MQ=255)                                                   cGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTg             <  2:387875/81‑1 (MQ=255)                                                       aaaGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAg         >  1:387921/1‑81 (MQ=255)                                                       aaaGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAg         >  1:387894/1‑81 (MQ=255)                                                       aaaGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAg         <  1:387876/81‑1 (MQ=255)                                                         aGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTc       >  1:387900/1‑81 (MQ=255)                                                           gTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTcca     >  1:387898/1‑81 (MQ=255)                                                              aTGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTccacca  >  1:387902/1‑81 (MQ=255)                                                                      |                                                                         AATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCA  >  NC_000913/2450947‑2451088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑