Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,477,517	T→C	E38G (GAA→GGA)	dsdC ←	DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,477,517	0	T	C	100.0%	77.7 / NA	23	E38G (GAA→GGA)	dsdC	DNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/12);  total (11/12)

GAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTA  >  NC_000913/2477440‑2477594                                                                              |                                                                              gaacAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCct                                                                            >  1:392024/1‑81 (MQ=255)         aacTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTgccgcc                                                                    <  1:392002/81‑1 (MQ=255)         aacTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTgccgc                                                                     >  2:392030/1‑80 (MQ=255)           cTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAg                                                                  <  1:392003/81‑1 (MQ=255)                   ccAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAgg                                                          <  2:392004/81‑1 (MQ=255)                   ccAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAg                                                           >  1:392028/1‑80 (MQ=255)                      aTTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACt                                                       >  2:392033/1‑81 (MQ=255)                         cttcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGAt                                                    >  2:392035/1‑81 (MQ=255)                                      attgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGGAGGACTGATGCCTGGCAGCCa                                        <  2:392007/80‑1 (MQ=255)                                      attgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCAc                                       <  1:392006/81‑1 (MQ=255)                                      attgatACGGTGACTTACCGCACTGGGACTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCAc                                       <  1:392005/81‑1 (MQ=255)                                         gatACGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACtt                                     >  2:392038/1‑80 (MQ=255)                                             cGGTGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCaaaa                                <  2:392008/81‑1 (MQ=255)                                                tGACTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTa                             >  2:392034/1‑81 (MQ=255)                                                   cTTACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGc                          <  1:392009/81‑1 (MQ=255)                                                    ttACCGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCa                         >  2:392039/1‑81 (MQ=255)                                                       ccGCACTGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCAttt                      >  2:392050/1‑81 (MQ=255)                                                             tGGGGCTCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGAt                 >  2:392032/1‑80 (MQ=255)                                                                   tCAGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGc           >  1:392031/1‑80 (MQ=255)                                                                     aGCGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGcc          <  1:392010/79‑1 (MQ=255)                                                                       cGACAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGcc      <  2:392011/81‑1 (MQ=255)                                                                          cAATCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGtt   <  1:392012/81‑1 (MQ=255)                                                                           aaTCCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTa  <  2:392013/81‑1 (MQ=255)                                                                              |                                                                              GAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTA  >  NC_000913/2477440‑2477594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑