Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,489,635	G→A	I184I (ATC→ATT)	yfdV ←	putative transport protein YfdV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,489,635	0	G	A	100.0%	69.1 / NA	21	I184I (ATC→ATT)	yfdV	putative transport protein YfdV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/14);  total (7/14)

CCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTATTCTT  >  NC_000913/2489559‑2489709                                                                             |                                                                           ccccTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCg                                                                        <  2:393711/81‑1 (MQ=255)   ccTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGtt                                                                      >  2:393742/1‑81 (MQ=255)        gTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAg                                                                 <  1:393712/81‑1 (MQ=255)          tAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGaa                                                               >  1:393749/1‑81 (MQ=255)              cTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAgg                                                           >  2:393753/1‑81 (MQ=255)               ttCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGt                                                          >  2:393755/1‑81 (MQ=255)                           ttAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATactact                                              <  1:393713/81‑1 (MQ=255)                                  ttGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATAGTTGACAGAAAAGGTGAAAATAATACTGGCTCCt                                       <  1:393714/81‑1 (MQ=255)                                    gggTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCttt                                     >  2:393752/1‑81 (MQ=255)                                    gggTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCtt                                      <  2:393715/80‑1 (MQ=255)                                         ccATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCg                                 >  1:393759/1‑80 (MQ=255)                                              ctgcCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGaa                            <  2:393717/80‑1 (MQ=255)                                                            aCCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAg              <  2:393718/80‑1 (MQ=255)                                                             ccccAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAgct            <  1:393720/81‑3 (MQ=255)                                                             ccccAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGAt            <  1:393719/81‑1 (MQ=255)                                                                 aaccaacACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACt        <  2:393721/81‑1 (MQ=255)                                                                 aaccaacACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACt        <  2:393722/81‑1 (MQ=255)                                                                  accaacACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTa       <  2:393723/81‑1 (MQ=255)                                                                    caacACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTAtt     <  1:393724/81‑1 (MQ=255)                                                                     aacACCAGAATCGTTGTCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTAttc    <  2:393725/81‑1 (MQ=255)                                                                       cacCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTAttctt  >  1:393758/1‑81 (MQ=255)                                                                             |                                                                           CCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTATTCTT  >  NC_000913/2489559‑2489709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑