Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,498,376	T→C	intergenic (‑81/‑295)	alaC ← / → ypdA	glutamate‑‑pyruvate aminotransferase AlaC/sensory histidine kinase YpdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,498,376	0	T	C	100.0%	68.3 / NA	20	intergenic (‑81/‑295)	alaC/ypdA	glutamate‑‑pyruvate aminotransferase AlaC/sensory histidine kinase YpdA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/13);  total (7/13)

TTTCCTCAGATTAACGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATTAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGAGGAACTCTGCT  >  NC_000913/2498298‑2498438                                                                               |                                                               tttCCTCAGATTAACGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCaa                                                              >  1:395059/1‑81 (MQ=255)   tCCTCAGATTAACGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAAtt                                                            >  1:395064/1‑81 (MQ=255)   tCCTCAGATTAACGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAAt                                                             <  1:395037/80‑1 (MQ=255)    ccTCAGATTAACGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAAttt                                                           >  1:395065/1‑81 (MQ=255)        aGATTAACGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAATTtctgt                                                       >  1:395063/1‑81 (MQ=255)           ttAACGTTAGCGCCCGGACAGTAAGAGCGACGCTGAAACGATGGTGGATGTTTTGAAAATAGCCTGATCAATTtctgtctg                                                    <  1:395038/81‑1 (MQ=255)             aaCGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAATTTctgtctgt                                                   <  1:395039/80‑1 (MQ=255)              aCGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAATTTCagtctgtc                                                  <  2:395040/80‑1 (MQ=255)                      gcCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGttt                                          >  1:395060/1‑80 (MQ=255)                                tCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACAtttt                               <  1:395041/81‑1 (MQ=255)                                    aGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTttatta                           <  1:395042/81‑1 (MQ=255)                                              ccACGATGGTGGCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTaa                 <  2:395043/81‑1 (MQ=255)                                                  gATGGTGGCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAAt               >  1:395062/1‑79 (MQ=255)                                                  gATGGTGGCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGa             <  1:395044/81‑1 (MQ=255)                                                    tggtggCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGAgg           <  1:395045/81‑1 (MQ=255)                                                     ggtggCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGAgg           >  1:395067/1‑80 (MQ=255)                                                      gtggCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGAGGa          <  2:395046/80‑1 (MQ=255)                                                         gCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGAGGAAct       <  2:395047/80‑1 (MQ=255)                                                         gCTGTTTTGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGAGGAAct       <  2:395048/80‑1 (MQ=255)                                                             ttttGAAAATAGCCTGATCAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGAGGAACTCTGCt  <  1:395049/81‑1 (MQ=255)                                                                               |                                                               TTTCCTCAGATTAACGTTAGCGCCCGGACCGTCCGAGCGACGCTGCCACGATGGTGGCTGTTTTGAAAATAGCCTGATTAATTTCTGTCTGTCCAGCGTTTTAGAACATTTTATTAATGACGTTAAATGAGGAACTCTGCT  >  NC_000913/2498298‑2498438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑