Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,498,790	C→T	H40H (CAC→CAT)	ypdA →	sensory histidine kinase YpdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,498,790	0	C	T	100.0%	70.0 / NA	20	H40H (CAC→CAT)	ypdA	sensory histidine kinase YpdA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

CAGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCC  >  NC_000913/2498714‑2498865                                                                             |                                                                            cAGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTccc                                                                         >  2:395112/1‑81 (MQ=255)  aGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTccc                                                                         >  2:395094/1‑80 (MQ=255)           gCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAAtt                                                               >  2:395111/1‑81 (MQ=255)                tCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTc                                                           <  2:395080/80‑1 (MQ=255)                tCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTc                                                           <  2:395079/80‑1 (MQ=255)                      tGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTa                                                    >  2:395105/1‑81 (MQ=255)                        ttctttctCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTAcc                                                  <  2:395081/81‑1 (MQ=255)                         tctttctCATCCGTATCCGCCTGTTTCGCAAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCg                                                 >  2:395114/1‑81 (MQ=255)                             tctcATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGcc                                               <  2:395082/79‑1 (MQ=255)                                cATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCAtttt                                          >  1:395110/1‑81 (MQ=255)                                  tCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTtct                                        >  1:395113/1‑81 (MQ=255)                                           gCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCg                               >  1:395108/1‑81 (MQ=255)                                                   cgcgAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGtt                        <  1:395083/80‑1 (MQ=255)                                                    gcgAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTc                       <  2:395084/80‑1 (MQ=255)                                                     cgAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAg                     >  1:395106/1‑81 (MQ=255)                                                        accgTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCAc                  <  2:395085/78‑1 (MQ=255)                                                          tGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCt                >  1:395109/1‑81 (MQ=255)                                                               cacaAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTc            <  1:395086/80‑1 (MQ=255)                                                                    gTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGt      <  2:395087/81‑1 (MQ=255)                                                                        gcgcATTCCCCAAAAGAATTGCTCACCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTccc  >  2:395117/1‑81 (MQ=255)                                                                             |                                                                            CAGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCC  >  NC_000913/2498714‑2498865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑