Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,070	T→C	V119A (GTA→GCA)	nhaR →	DNA‑binding transcriptional activator NhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,070	0	T	C	100.0%	139.6 / NA	41	V119A (GTA→GCA)	nhaR	DNA‑binding transcriptional activator NhaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/23);  total (18/23)

CAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAAC  >  NC_000913/18993‑19147                                                                              |                                                                              cAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAg                                                                             <  1:4037/80‑1 (MQ=255) cAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGa                                                                            <  2:4036/81‑1 (MQ=255)    tttATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAgg                                                                         <  1:4038/81‑1 (MQ=255)      tATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGc                                                                        <  1:4039/80‑1 (MQ=255)      tATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGc                                                                        <  1:4040/80‑1 (MQ=255)      tATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGc                                                                        >  2:4089/1‑80 (MQ=255)       aTTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGc                                                                        >  2:4078/1‑79 (MQ=255)       aTTGTTTGACGTCGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGc                                                                        >  2:4075/1‑79 (MQ=255)          gTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGc                                                                    <  2:4041/80‑1 (MQ=255)           tttGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGccc                                                                  <  2:4043/81‑1 (MQ=255)           tttGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGcc                                                                   <  2:4042/80‑1 (MQ=255)            ttGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCa                                                                 >  2:4095/1‑81 (MQ=255)                 gTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCAt                                                            >  1:4093/1‑81 (MQ=255)                   tGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCt                                                          >  2:4115/1‑81 (MQ=255)                     gCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTc                                                        <  2:4045/81‑1 (MQ=255)                     gCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTc                                                        <  2:4044/81‑1 (MQ=255)                     gCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTc                                                        >  1:4105/1‑81 (MQ=255)                      cGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCg                                                       >  1:4094/1‑81 (MQ=255)                          gCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCgctgc                                                   <  2:4046/81‑1 (MQ=255)                             gATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTc                                                >  2:4085/1‑81 (MQ=255)                              aTGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCtt                                                 <  2:4047/79‑1 (MQ=255)                                 cACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAAt                                            <  2:4049/81‑1 (MQ=255)                                 cACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAAt                                            <  1:4048/81‑1 (MQ=255)                                   cTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATcc                                          <  1:4050/81‑1 (MQ=255)                                      tCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATccac                                        <  2:4051/80‑1 (MQ=255)                                        cAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATccaccca                                     >  2:4104/1‑81 (MQ=255)                                        cAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATccaccc                                      <  1:4052/80‑1 (MQ=255)                                          aaCGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATccacccac                                    >  2:4091/1‑80 (MQ=255)                                              ccTGGTCAGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAAt                               <  2:4053/81‑1 (MQ=255)                                                     aGTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGctgctg                        >  2:4109/1‑81 (MQ=255)                                                      gTAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTgg                       <  2:4054/81‑1 (MQ=255)                                                       tAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGctgctg                        >  2:4100/1‑79 (MQ=255)                                                       tAGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGa                      <  1:4055/81‑1 (MQ=255)                                                        aGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAg                     >  1:493987/1‑81 (MQ=255)                                                        aGCGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAg                     >  1:4096/1‑81 (MQ=255)                                                          cGTACTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGc                    >  2:4099/1‑80 (MQ=255)                                                             aCTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAAt                 >  2:4116/1‑80 (MQ=255)                                                             aCTTAACGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAAt                 >  2:4106/1‑80 (MQ=255)                                                                  aCGCCGCAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTc           <  1:4056/81‑1 (MQ=255)                                                                    gccgcAGTGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAg         <  2:4057/81‑1 (MQ=255)                                                                           tGGCAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAAc  <  1:4058/81‑1 (MQ=255)                                                                              |                                                                              CAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTGGTCAGTAGCGTACTTAACGCCGCAGTGGTAGAAGGCGAGCCCATTCATCTTCGCTGCTTCGAATCCACCCACGAAATGCTGCTGGAGCAATTAAGTCAGCATAAAC  >  NC_000913/18993‑19147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑