Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	267,012	A→G	intergenic (‑459/+172)	yafY ← / ← yafZ	CP4‑6 prophage; inner membrane lipoprotein YafY/CP4‑6 prophage; conserved protein YafZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	267,012	0	A	G	100.0%	89.1 / NA	26	intergenic (‑459/+172)	yafY/yafZ	CP4‑6 prophage; inner membrane lipoprotein YafY/CP4‑6 prophage; conserved protein YafZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

CAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCG  >  NC_000913/266935‑267087                                                                              |                                                                            cagccGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCtt                                                                          >  2:56285/1‑81 (MQ=255) cagccGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCtt                                                                          <  1:56235/81‑1 (MQ=255)  agccGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCttt                                                                         <  1:56236/81‑1 (MQ=255)     cGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTa                                                                        >  1:56268/1‑79 (MQ=255)      ggTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAag                                                                     <  2:56237/81‑1 (MQ=255)                gcgTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCg                                                            >  1:56279/1‑80 (MQ=255)                  gTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGa                                                          >  2:56280/1‑80 (MQ=255)                        ccTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGt                                                    <  1:56238/80‑1 (MQ=255)                        ccTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGt                                                    <  2:56239/80‑1 (MQ=255)                           gtgtCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATg                                                 >  1:56277/1‑80 (MQ=255)                                   agaCGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGAcaccac                                         <  2:56240/80‑1 (MQ=255)                                       gTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGaaa                                    >  1:56273/1‑81 (MQ=255)                                        ttCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAAc                                   <  1:56241/81‑1 (MQ=255)                                          ctcCGTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTa                                 >  2:56281/1‑81 (MQ=255)                                              gTGAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTAtttt                             >  2:56283/1‑81 (MQ=255)                                                gAAAGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTAttttt                            >  2:56288/1‑80 (MQ=255)                                                 aaaGTTAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTc                           <  1:56242/80‑1 (MQ=255)                                                      tAACTGACTGAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCt                     >  1:56286/1‑81 (MQ=255)                                                        actgactgAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGc                   <  1:56244/81‑1 (MQ=255)                                                        actgactgAAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGc                   <  1:56243/81‑1 (MQ=255)                                                            actgaAAATGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCaa                 <  2:56245/79‑1 (MQ=255)                                                                aaaaTGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGc             >  2:56276/1‑79 (MQ=255)                                                                aaaaTGATGTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCa           <  1:56246/81‑1 (MQ=255)                                                                    tgatgTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAgg       <  2:56247/81‑1 (MQ=255)                                                                      atgTGATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCa     <  2:56248/81‑1 (MQ=255)                                                                         tgATTGCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCg  <  2:56249/81‑1 (MQ=255)                                                                              |                                                                            CAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCG  >  NC_000913/266935‑267087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑