Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,517,703	G→A	L44L (CTG→TTG)	pdeA ←	putative c‑di‑GMP phosphodiesterase PdeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,517,703	0	G	A	100.0%	71.0 / NA	21	L44L (CTG→TTG)	pdeA	putative c‑di‑GMP phosphodiesterase PdeA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/11);  total (10/11)

AACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAA  >  NC_000913/2517632‑2517780                                                                        |                                                                              aaCGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGAtata                                                                      <  1:398534/81‑1 (MQ=255)      gAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTgg                                                                 <  1:398535/81‑1 (MQ=255)           acacCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATg                                                             >  2:398551/1‑80 (MQ=255)             acCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGAt                                                          <  2:398536/81‑1 (MQ=255)                gcgcCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAAt                                                       >  1:398555/1‑81 (MQ=255)                  gccagccaAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATgg                                                     >  1:398558/1‑81 (MQ=255)                       ccaAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGt                                                <  2:398537/81‑1 (MQ=255)                          aaGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGc                                             >  2:398564/1‑81 (MQ=255)                                    aGATTGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAAc                                   <  2:398538/81‑1 (MQ=255)                                       ttGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACggg                                <  2:398539/81‑1 (MQ=255)                                       ttGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACgg                                 >  2:398557/1‑80 (MQ=255)                                        tGACAGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGa                               >  2:398579/1‑81 (MQ=255)                                            aGCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGc                           >  2:398561/1‑81 (MQ=255)                                             gCATTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCt                          <  2:398540/81‑1 (MQ=255)                                               aTTACGCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTg                         >  2:398549/1‑80 (MQ=255)                                                    gCACAGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAg                   >  1:398563/1‑81 (MQ=255)                                                        aGTGGCATCCACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTAc                >  2:398560/1‑80 (MQ=255)                                                                 cACGCCAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGc      <  1:398541/81‑1 (MQ=255)                                                                   cGCCAAAAAGATATACCTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGCTAGCTGAATAGGTACCACGGTGAGCGt    <  2:398542/81‑1 (MQ=255)                                                                     ccAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTaa  <  1:398544/81‑1 (MQ=255)                                                                     ccAAAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTaa  <  1:398543/81‑1 (MQ=255)                                                                        |                                                                              AACGGGAACGACACCGCGCCAGCCAAAGATAAACAAGATTGACAGCATTACGCACAGTGGCATCCACGCCAGAAAGATATAACTGGAATGGATAATGGCAAGTGGCGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAA  >  NC_000913/2517632‑2517780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑