Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,520,945	G→A	noncoding (15/76 nt)	valU →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,520,945	0	G	A	100.0%	96.7 / NA	28	noncoding (15/76 nt)	valU	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/9);  total (19/9)

AATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATG  >  NC_000913/2520867‑2521017                                                                               |                                                                         aaTTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACt                                                                        >  1:399103/1‑81 (MQ=255)          aGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCa                                                               >  1:399112/1‑81 (MQ=255)          aGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCa                                                               >  1:399111/1‑81 (MQ=255)             aaaTGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGATAGCACCt                                                            >  1:399118/1‑81 (MQ=255)                 gCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCt                                                        >  2:399113/1‑81 (MQ=255)                 gCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCt                                                        >  2:399109/1‑81 (MQ=255)                  cGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCtt                                                       <  1:399073/81‑1 (MQ=255)                           aTTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAaggagg                                              >  2:399099/1‑81 (MQ=255)                           aTTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAaggagg                                              >  1:399095/1‑81 (MQ=255)                            ttttGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAggg                                             >  1:399106/1‑81 (MQ=255)                                 aaCTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTc                                         <  1:399074/80‑1 (MQ=255)                                  aCTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcgg                                       >  2:399107/1‑81 (MQ=255)                                   ctctCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggc                                      >  1:399100/1‑81 (MQ=255)                                   ctctCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggc                                      <  1:399075/81‑1 (MQ=255)                                     ctcCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcgg                                    >  2:399117/1‑81 (MQ=255)                                     ctcCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcgg                                    >  1:399130/1‑81 (MQ=255)                                        ccTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTc                                 <  1:399076/81‑1 (MQ=255)                                         cTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCg                                <  1:399077/81‑1 (MQ=255)                                              aTGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                            <  2:399078/80‑1 (MQ=255)                                                  gACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtca                       >  1:399124/1‑81 (MQ=255)                                                   aCTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                      <  1:399079/81‑1 (MQ=255)                                                    cTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                      >  2:399104/1‑80 (MQ=255)                                                       cacacaGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacc                  <  2:399080/81‑1 (MQ=255)                                                        acacaGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccc                 <  1:399081/81‑1 (MQ=255)                                                          acaGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacccac               >  1:399082/1‑81 (MQ=255)                                                            aGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacccacc              >  1:399129/1‑80 (MQ=255)                                                                      aTTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTAt   >  1:399140/1‑81 (MQ=255)                                                                        tAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCTACTTTATg  >  2:399135/1‑80 (MQ=255)                                                                               |                                                                         AATTTCTTTAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATG  >  NC_000913/2520867‑2521017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑