Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,526,860	T→C	intergenic (‑4/‑86)	yfeR ← / → yfeH	putative LysR‑type DNA‑binding transcriptional regulator YfeR/putative solute:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,526,860	0	T	C	100.0%	70.4 / NA	21	intergenic (‑4/‑86)	yfeR/yfeH	putative LysR‑type DNA‑binding transcriptional regulator YfeR/putative solute:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/13);  total (8/13)

CACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGTTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAGAACCATCGATCAT  >  NC_000913/2526790‑2526934                                                                       |                                                                           cACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCAt                                                                   <  2:400062/80‑1 (MQ=255)  aCGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGa                                                                 <  2:400063/81‑1 (MQ=255)      cTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATgg                                                             <  1:400064/81‑1 (MQ=255)          aaCTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGATTAATTCATCTGCTTTCTTCATGAATGGATg                                                          >  2:400105/1‑80 (MQ=255)                 ctcCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAAt                                                  >  1:400094/1‑81 (MQ=255)                               tgACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTtc                                      <  2:400066/79‑1 (MQ=255)                               tgACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTtc                                      <  2:400065/79‑1 (MQ=255)                                 aCGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCaa                                  <  2:400067/81‑1 (MQ=255)                                    aaaaCCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACt                               >  2:400088/1‑81 (MQ=255)                                      aaCCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTgg                             <  2:400068/81‑1 (MQ=255)                                      aaCCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTgg                             <  2:400069/81‑1 (MQ=255)                                       aCCTTTAATTGTTTTAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGga                            <  2:400070/81‑1 (MQ=255)                                                  ttttAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACaa                  >  2:400090/1‑80 (MQ=255)                                                  ttttAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAAt                 <  1:400071/81‑1 (MQ=255)                                                     tAAAGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAg               <  1:400072/80‑1 (MQ=255)                                                       aaGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAGAAc            <  1:400073/81‑1 (MQ=255)                                                        aGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAGAAcc           <  2:400074/81‑1 (MQ=255)                                                        aGAATAATTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAGAAc            >  2:400095/1‑80 (MQ=255)                                                             aatTCATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAGAACCATCGa      >  1:400102/1‑81 (MQ=255)                                                                 cATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAGAACCATCGatcat  >  2:400092/1‑81 (MQ=255)                                                                 cATCTGCTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAGAACCATCGatcat  >  2:400098/1‑81 (MQ=255)                                                                       |                                                                           CACGACTAAAACTTTTCTCCTGCGCTACTGTGACGAAAACCTTTAATTGTTTTAAAGAATAATTCATCTGTTTTCTTCATGAATGGATGCAATAAATCAATTTTATTTCTCAAACTGGAAGAAGCACAATAGAACCATCGATCAT  >  NC_000913/2526790‑2526934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑