Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,588,353	C→T	V253V (GTC→GTT)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,588,353	0	C	T	100.0%	118.2 / NA	33	V253V (GTC→GTT)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/19);  total (14/19)

GCAGGCGCTCAACGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTCAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCGGAGA  >  NC_000913/2588275‑2588414                                                                               |                                                              gCATGCGCTCAACGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTaa                                                             >  1:411636/1‑81 (MQ=255)   aGGCGCTCAACGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATc                                                           <  2:411546/81‑1 (MQ=255)   aGGCGCTCAACGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATc                                                           <  2:411545/81‑1 (MQ=255)      cgcTCAACGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAgg                                                        <  2:411547/81‑1 (MQ=255)        cTCAACGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGAc                                                      <  1:411548/81‑1 (MQ=255)          cAACGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACgg                                                    >  2:411589/1‑81 (MQ=255)           aaCGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGc                                                   <  1:411549/81‑1 (MQ=255)           aaCGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGc                                                   >  2:411593/1‑81 (MQ=255)             cGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTc                                                 <  2:411550/81‑1 (MQ=255)              gCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCTCGATATCACCTTTCGGGTTAATCAGTACGGCt                                                  >  1:411566/1‑79 (MQ=255)                    aTTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGt                                           >  1:411596/1‑80 (MQ=255)                       aaCGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAgg                                       >  2:411590/1‑81 (MQ=255)                       aaCGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCCCAGAGGTAAgg                                       >  1:411586/1‑81 (MQ=255)                            ccAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTggg                                  >  2:411605/1‑81 (MQ=255)                             cAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGc                                 <  1:411551/81‑1 (MQ=255)                                tCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGAt                              <  1:411552/81‑1 (MQ=255)                                  aCTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGt                            <  1:411553/81‑1 (MQ=255)                                         cAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCAcc                     <  2:411554/81‑1 (MQ=255)                                          aaaCACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCg                    <  1:411555/81‑1 (MQ=255)                                           aaCACCAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGt                   >  2:411598/1‑81 (MQ=255)                                               ccAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGaa               <  1:411556/81‑1 (MQ=255)                                                cAGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGaaa              <  1:411557/81‑1 (MQ=255)                                                 aGAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAAt             <  2:411558/81‑1 (MQ=255)                                                  gAACAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATg            >  2:411611/1‑81 (MQ=255)                                                   aaCAGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATgg           <  1:411559/81‑1 (MQ=255)                                                      aGTTCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATggggg        >  2:411600/1‑81 (MQ=255)                                                        ttCCGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCg      <  1:411560/81‑1 (MQ=255)                                                          ccGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCgg     <  2:411561/80‑1 (MQ=255)                                                          ccGCGATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCg      >  2:411595/1‑79 (MQ=255)                                                           cgcgATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCGgag   <  1:411563/81‑1 (MQ=255)                                                           cgcgATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCGga    <  1:411562/80‑1 (MQ=255)                                                            gcgATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCGgag   >  2:411594/1‑80 (MQ=255)                                                             cgATATCACCTTGCGGGTTAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCGgaga  >  1:411613/1‑80 (MQ=255)                                                                               |                                                              GCAGGCGCTCAACGCCACCATTAACGCCCAGTCACTGCTGCAAACACCAGAACAGTTCCGCGATATCACCTTGCGGGTCAATCAGGACGGCTCAGAGGTAAGGCTGGGCGATGTCGCCACCGTCGAAATGGGGGCGGAGA  >  NC_000913/2588275‑2588414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑