Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,597,902	T→C	K322E (AAA→GAA)	bamC ←	outer membrane protein assembly factor BamC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,597,902	0	T	C	100.0%	95.1 / NA	28	K322E (AAA→GAA)	bamC	outer membrane protein assembly factor BamC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/8);  total (20/8)

TTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCT  >  NC_000913/2597831‑2597978                                                                        |                                                                             ttACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGAt                                                                     >  2:413380/1‑81 (MQ=255)  tACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGAt                                                                     <  1:413358/80‑1 (MQ=255)  tACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGAt                                                                     >  2:413389/1‑80 (MQ=255)  tACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGAt                                                                     >  2:413385/1‑80 (MQ=255)  tACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGAt                                                                     <  2:413359/80‑1 (MQ=255)      tGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACt                                                                >  1:413397/1‑81 (MQ=255)       gCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACt                                                                <  2:413360/80‑1 (MQ=255)           aaCGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAgg                                                           >  2:413406/1‑81 (MQ=255)                 gCCTGGAAGACAGCTACCAGCGCGTCGTTCTAACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGctgctg                                                     <  2:413361/81‑1 (MQ=255)                   cTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCg                                                   >  1:413387/1‑81 (MQ=255)                      gAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCgg                                                  <  2:413362/79‑1 (MQ=255)                       aaGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTa                                               >  2:413400/1‑81 (MQ=255)                       aaGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTa                                               >  1:413405/1‑81 (MQ=255)                         gACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATc                                             <  2:413363/81‑1 (MQ=255)                                  cAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGc                                     >  2:413392/1‑80 (MQ=255)                                   aGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCa                                   <  1:413364/81‑1 (MQ=255)                                       cgtcgtTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCt                               >  2:413394/1‑81 (MQ=255)                                          cgtTCTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTg                              >  1:413388/1‑79 (MQ=255)                                              cTGACTCTGAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGtt                         >  1:413395/1‑80 (MQ=255)                                                      gAGTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCAc                >  1:413402/1‑81 (MQ=255)                                                        gTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCg              >  2:413407/1‑81 (MQ=255)                                                        gTCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCg              >  2:413413/1‑81 (MQ=255)                                                         tCAGAGTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCgg             <  2:413365/81‑1 (MQ=255)                                                           agagTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGAt           >  2:413393/1‑81 (MQ=255)                                                           agagTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGAt           >  2:413417/1‑81 (MQ=255)                                                              gTATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc         >  2:413404/1‑80 (MQ=255)                                                               tATGACCTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGc         >  1:413425/1‑79 (MQ=255)                                                                    ccTTCCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCt  >  1:413403/1‑81 (MQ=255)                                                                        |                                                                             TTACTTGCTAAACGCAGCCTGGAAGACAGCTACCAGCGCGTCGTTCTGACTCTGAGTCAGAGTATGACCTTTCGGATCGATGAACTGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCT  >  NC_000913/2597831‑2597978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑