Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,601,519	G→A	A107T (GCC→ACC)	hyfA →	hydrogenase 4 component A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,601,519	0	G	A	100.0%	117.8 / NA	33	A107T (GCC→ACC)	hyfA	hydrogenase 4 component A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/15);  total (18/15)

CTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTG  >  NC_000913/2601441‑2601596                                                                               |                                                                              ctctGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATAcc                                                                             <  2:414023/81‑1 (MQ=255) ctctGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATAcc                                                                             <  1:414024/81‑1 (MQ=255) ctctGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATAcc                                                                             >  1:414074/1‑81 (MQ=255)    tGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATAccc                                                                            <  1:414025/79‑1 (MQ=255)     gTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATg                                                                         >  1:414060/1‑81 (MQ=255)          ggCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCaa                                                                    >  2:414058/1‑81 (MQ=255)          ggCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCaa                                                                    >  1:414065/1‑81 (MQ=255)            cTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAata                                                                  >  1:414062/1‑81 (MQ=255)            cTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAata                                                                  >  1:414070/1‑81 (MQ=255)              gCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATg                                                                >  1:414067/1‑81 (MQ=255)               cAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAatat                                                                 <  2:414026/79‑1 (MQ=255)                aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGtt                                                              >  2:414068/1‑81 (MQ=255)                aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGtt                                                              >  2:414077/1‑81 (MQ=255)                  gCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGtttt                                                            <  1:414027/81‑1 (MQ=255)                     ttGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGttttt                                                           >  2:414081/1‑79 (MQ=255)                       gcgcCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAg                                                        <  1:414029/80‑1 (MQ=255)                       gcgcCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAg                                                        <  2:414028/80‑1 (MQ=255)                          ccGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTg                                                    <  2:414030/81‑1 (MQ=255)                                   gCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGctcac                                           >  1:414076/1‑81 (MQ=255)                                    cccATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGctcact                                          >  1:414082/1‑81 (MQ=255)                                     ccATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGctcactc                                         <  2:414031/81‑1 (MQ=255)                                         ttGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGctcactca                                        >  2:414089/1‑78 (MQ=255)                                                aaTCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCga                              <  2:414032/81‑1 (MQ=255)                                                aaTCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCg                               >  1:414073/1‑80 (MQ=255)                                                aaTCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCg                               >  1:414071/1‑80 (MQ=255)                                                    aGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCgaaga                           >  1:414080/1‑80 (MQ=255)                                                    aGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCgaaga                           <  2:414033/80‑1 (MQ=255)                                                     gcgcTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCgaagaa                          >  2:414091/1‑80 (MQ=255)                                                           cAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAAcgc                     <  1:414034/79‑1 (MQ=255)                                                            aGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACgcgc                   <  1:414035/80‑1 (MQ=255)                                                                aGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaaca              >  1:414085/1‑81 (MQ=255)                                                                         gTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCt     <  2:414036/81‑1 (MQ=255)                                                                            aaTACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTtg  <  1:414037/81‑1 (MQ=255)                                                                               |                                                                              CTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTG  >  NC_000913/2601441‑2601596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑