Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	268,409	C→T	E184E (GAG→GAA)	ykfA ←	CP4‑6 prophage; putative GTP‑binding protein YkfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	268,409	0	C	T	100.0%	96.2 / NA	27	E184E (GAG→GAA)	ykfA	CP4‑6 prophage; putative GTP‑binding protein YkfA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/19);  total (8/19)

ATCCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGCTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGTTC  >  NC_000913/268332‑268486                                                                              |                                                                              atcCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCa                                                                            <  2:56535/81‑1 (MQ=255)  tcCCCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCAt                                                                           <  1:56536/81‑1 (MQ=255)    cGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATgc                                                                         <  1:56537/81‑1 (MQ=255)         ggCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGcagcag                                                                    <  2:56538/81‑1 (MQ=255)           ccATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAgcaggc                                                                  <  1:56539/81‑1 (MQ=255)            cATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAgcaggca                                                                 <  1:56540/81‑1 (MQ=255)                 ccTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGAttt                                                            <  1:56541/81‑1 (MQ=255)                      aGCCCCCATTGTAGACTGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCgg                                                       >  1:56592/1‑81 (MQ=255)                      aGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCg                                                        <  1:56542/80‑1 (MQ=255)                       gCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGc                                                      >  2:56591/1‑81 (MQ=255)                         ccccATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTg                                                    <  1:56543/81‑1 (MQ=255)                           ccATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGa                                                   >  2:56597/1‑80 (MQ=255)                            cATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGata                                                 <  1:56544/81‑1 (MQ=255)                                       gACCGACACAGCACACACCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATAttctgcttctg                                      <  2:56545/81‑1 (MQ=255)                                                 gCACACGCCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGAc                            <  2:56546/81‑1 (MQ=255)                                                  cacacaCCGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACa                           <  2:56547/81‑1 (MQ=255)                                                        ccGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTc                     <  1:56548/81‑1 (MQ=255)                                                         cGGATGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTcc                    >  2:56594/1‑81 (MQ=255)                                                             tGGACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCa                >  1:56603/1‑81 (MQ=255)                                                               gACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCAcc              >  1:56593/1‑81 (MQ=255)                                                               gACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCAcc              <  2:56550/81‑1 (MQ=255)                                                               gACGGGCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCAcc              <  1:56549/81‑1 (MQ=255)                                                                  gggCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCt           >  1:56587/1‑81 (MQ=255)                                                                   ggCTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTg          >  2:56596/1‑81 (MQ=255)                                                                     cTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGt        <  1:56552/81‑1 (MQ=255)                                                                     cTGGAACAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGt        <  2:56551/81‑1 (MQ=255)                                                                           cAGTTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGTTc  <  1:56553/81‑1 (MQ=255)                                                                              |                                                                              ATCCGCTCGGCCATCACCTTAAGCCCCCATTGTAGACGGACCGACACAGCACACACCGGATGGACGGGCTGGAACAGCTCATGCAGCAGGCAGATTTTGCGGCTGATATTCTGCTTCTGCGCCGTGGACAACTGTCCCCCACCGCTGGTAGGTTC  >  NC_000913/268332‑268486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑