Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,607,268	T→C	F118L (TTC→CTC)	hyfF →	hydrogenase 4 component F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,607,268	0	T	C	100.0%	85.5 / NA	25	F118L (TTC→CTC)	hyfF	hydrogenase 4 component F
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

TTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATC  >  NC_000913/2607192‑2607342                                                                             |                                                                           ttACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTctctt                                                                        >  2:415220/1‑81 (MQ=255)       gATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATc                                                                   <  1:415194/80‑1 (MQ=255)           ggCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGttt                                                              >  2:415224/1‑81 (MQ=255)             cTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGttttt                                                            <  2:415195/81‑1 (MQ=255)                 aTGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGtt                                                         >  1:415221/1‑80 (MQ=255)                 aTGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTc                                                        <  2:415196/81‑1 (MQ=255)                  tGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCa                                                       >  1:415216/1‑81 (MQ=255)                      tCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCAt                                                   <  1:415197/81‑1 (MQ=255)                      tCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCAt                                                   <  2:415198/81‑1 (MQ=255)                        aTGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCAtgc                                                 >  1:415230/1‑81 (MQ=255)                          gAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCAtgctg                                               >  1:415229/1‑81 (MQ=255)                             gTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGctgctg                                            >  1:415234/1‑81 (MQ=255)                                    acGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTa                                     >  1:415219/1‑81 (MQ=255)                                        cGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAg                                 <  2:415199/81‑1 (MQ=255)                                         gAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAg                                 >  2:415223/1‑80 (MQ=255)                                              ttCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATaa                           >  2:415236/1‑81 (MQ=255)                                               tCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTCCACCATGCTGCTGGTTGTTACCAGCAATAAc                          >  2:415232/1‑81 (MQ=255)                                                     gTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGAtt                    <  1:415200/81‑1 (MQ=255)                                                      ttACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATtg                   <  1:415201/81‑1 (MQ=255)                                                        aCGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATtgt                  >  2:415238/1‑80 (MQ=255)                                                        aCGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATtgt                  <  2:415202/80‑1 (MQ=255)                                                            tgtgCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGAtgt             >  1:415242/1‑81 (MQ=255)                                                                 gATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGggcg        >  1:415239/1‑81 (MQ=255)                                                                  aTTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGggcgg       <  1:415203/81‑1 (MQ=255)                                                                      ctacGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGAt   <  2:415204/81‑1 (MQ=255)                                                                        acGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATc  >  1:415240/1‑80 (MQ=255)                                                                             |                                                                           TTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATC  >  NC_000913/2607192‑2607342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑