Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,637,925	C→A	R243L (CGT→CTT)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,637,925	0	C	A	100.0%	144.1 / NA	40	R243L (CGT→CTT)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/16);  total (24/16)

GTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCG  >  NC_000913/2637850‑2638002                                                                            |                                                                              gTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCa                                                                          <  1:420831/81‑1 (MQ=255)   cAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAAt                                                                        >  1:420901/1‑81 (MQ=255)    aGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAAtt                                                                       >  1:420891/1‑81 (MQ=255)    aGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAAtt                                                                       >  1:420882/1‑81 (MQ=255)     ggTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAAttt                                                                      <  2:420832/81‑1 (MQ=255)     ggTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAAttt                                                                      >  1:420876/1‑81 (MQ=255)          ccATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTa                                                                 <  2:420833/81‑1 (MQ=255)            aTTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGa                                                               <  1:420834/81‑1 (MQ=255)            aTTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGa                                                               <  1:420835/81‑1 (MQ=255)               ataGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAAcc                                                            >  1:420871/1‑81 (MQ=255)               ataGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAAc                                                             <  2:420836/80‑1 (MQ=255)                  ggCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGt                                                         >  2:420893/1‑81 (MQ=255)                   gCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTc                                                        <  1:420837/81‑1 (MQ=255)                      aGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGcc                                                     >  1:420869/1‑81 (MQ=255)                         gcgAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTgg                                                  >  1:420908/1‑81 (MQ=255)                           gAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTggg                                                 >  1:420889/1‑80 (MQ=255)                            aaaaCAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGaa                                               <  2:420838/81‑1 (MQ=255)                              aacaacGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAAt                                             <  1:420839/81‑1 (MQ=255)                                 aacGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACg                                          >  1:420890/1‑81 (MQ=255)                                  acGCCGTTAACAACGACGGGAGTCGTGTCAACATAGCTAAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACgc                                         <  1:420840/81‑1 (MQ=255)                                        ttAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCtgctgc                                   <  2:420841/81‑1 (MQ=255)                                          aacaacGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGcc                                  >  1:420913/1‑80 (MQ=255)                                           acaacGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCa                                 >  2:420910/1‑80 (MQ=255)                                             aacGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAat                              <  2:420842/81‑1 (MQ=255)                                              acgacgGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAatc                             <  2:420843/81‑1 (MQ=255)                                              acgacgGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAat                              >  1:420896/1‑80 (MQ=255)                                              acgacgGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAat                              >  2:420897/1‑80 (MQ=255)                                                   gggAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTg                        >  2:420906/1‑81 (MQ=255)                                                   gggAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTg                        >  1:420892/1‑81 (MQ=255)                                                    ggAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTgg                       >  2:420905/1‑81 (MQ=255)                                                          gtgtCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGt                 >  1:420912/1‑81 (MQ=255)                                                           tgtCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGtt                <  2:420844/81‑1 (MQ=255)                                                             tCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTc               >  2:420895/1‑80 (MQ=255)                                                                  aTCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAg         >  1:420920/1‑81 (MQ=255)                                                                  aTCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAg         >  2:420903/1‑81 (MQ=255)                                                                   tCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGc        <  1:420845/81‑1 (MQ=255)                                                                    cGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCa       >  1:420885/1‑81 (MQ=255)                                                                     gCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCAc      <  1:420846/81‑1 (MQ=255)                                                                         aGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTgcg  >  1:420909/1‑81 (MQ=255)                                                                         aGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTgcg  >  1:420914/1‑81 (MQ=255)                                                                            |                                                                              GTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCG  >  NC_000913/2637850‑2638002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑