Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,669,174	A→G	H48R (CAC→CGC)	hcaE →	putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,669,174	0	A	G	100.0%	72.2 / NA	22	H48R (CAC→CGC)	hcaE	putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/10);  total (12/10)

GTATTTATACCGACCCGGATATTTACCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAG  >  NC_000913/2669099‑2669250                                                                            |                                                                             gTATTTATACCGACCCGGATATTTACCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGa                                                                           >  1:427007/1‑79 (MQ=255)           cGACCCGGATATTTACCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATccc                                                               <  2:426969/81‑1 (MQ=255)              cccGGATATTTACCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCaaa                                                            <  2:426970/81‑1 (MQ=255)                cGGATATTTACCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAAc                                                          <  1:426971/81‑1 (MQ=255)                         aCCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCTCGCGAAAGCCAGATCCCAAAACCCGGTGAtt                                                 >  2:427006/1‑81 (MQ=255)                         aCCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGAtt                                                 >  2:427004/1‑81 (MQ=255)                           cAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTc                                               <  2:426972/81‑1 (MQ=255)                             aCTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCtt                                             <  2:426973/81‑1 (MQ=255)                                 gAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTa                                           <  1:426974/79‑1 (MQ=255)                                        aGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTAc                                   <  1:426975/80‑1 (MQ=255)                                                ttCGGTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGgag                            >  1:427010/1‑79 (MQ=255)                                                   ggTCGTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGAt                       >  2:427011/1‑81 (MQ=255)                                                       gTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCgg                   <  2:426977/81‑1 (MQ=255)                                                       gTTGCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATAGGAGAAGATGCgg                   <  2:426976/81‑1 (MQ=255)                                                          gCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTg                >  1:427000/1‑81 (MQ=255)                                                          gCTGGCTATTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTg                >  2:426994/1‑81 (MQ=255)                                                                 aTTTCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGt         >  2:427009/1‑81 (MQ=255)                                                                  tttCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTg        >  1:427001/1‑81 (MQ=255)                                                                   ttCTCGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGc       >  2:427005/1‑81 (MQ=255)                                                                     ctcGCCCGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGt     >  2:427003/1‑81 (MQ=255)                                                                        gCCCGCGAAAGCCAGATCCCAAAACCCGGAGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTc    <  1:426978/79‑1 (MQ=255)                                                                         cccGCGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAg  >  1:426999/1‑80 (MQ=255)                                                                            |                                                                             GTATTTATACCGACCCGGATATTTACCAACTGGAGCTTGAGCGTATTTTCGGTCGTTGCTGGCTATTTCTCGCCCACGAAAGCCAGATCCCAAAACCCGGTGATTTCTTTAACACCTACATGGGAGAAGATGCGGTTGTCGTAGTGCGTCAG  >  NC_000913/2669099‑2669250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑