Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,672,278	G→A	G78S (GGT→AGT)	hcaD →	putative 3‑phenylpropionate/cinnamate dioxygenase ferredoxin reductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,672,278	0	G	A	100.0%	94.9 / NA	27	G78S (GGT→AGT)	hcaD	putative 3‑phenylpropionate/cinnamate dioxygenase ferredoxin reductase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

CTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACT  >  NC_000913/2672203‑2672347                                                                            |                                                                      cTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAgtgt                                                                   <  2:427546/80‑1 (MQ=255) cTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTa                                                                  <  1:427545/81‑1 (MQ=255)    gAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTaa                                                                 <  2:427547/79‑1 (MQ=255)      aGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCAt                                                             <  1:427548/81‑1 (MQ=255)        aTTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCa                                                           <  1:427549/81‑1 (MQ=255)         ttCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCaa                                                          <  1:427550/81‑1 (MQ=255)               gCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCt                                                    <  1:427551/81‑1 (MQ=255)               gCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCt                                                    <  2:427552/81‑1 (MQ=255)               gCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCt                                                    >  1:427584/1‑81 (MQ=255)                   ttACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGt                                                >  2:427580/1‑81 (MQ=255)                      cagcagGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTcgc                                             >  1:427590/1‑81 (MQ=255)                       agcagGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTcgcg                                            <  2:427553/81‑1 (MQ=255)                       agcagGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTcgc                                             >  1:427597/1‑80 (MQ=255)                          agGTGTTACACGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGaca                                         >  2:427588/1‑81 (MQ=255)                              gtTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGacacac                                      >  1:427583/1‑80 (MQ=255)                                    cGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAGAACGCTGGGTCGCGACACACGAGAGt                                >  2:427579/1‑80 (MQ=255)                                         aCTGGTGGCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAgtgt                          <  1:427554/81‑1 (MQ=255)                                              tggCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAAc                      >  1:427586/1‑80 (MQ=255)                                               ggCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAAcc                     >  1:427587/1‑80 (MQ=255)                                                gCAGGAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCaa                   >  1:427592/1‑81 (MQ=255)                                                   ggAAAACAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACgg                >  2:427605/1‑81 (MQ=255)                                                        aCAATGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGa             >  2:427607/1‑79 (MQ=255)                                                           aTGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCt        <  1:427556/81‑1 (MQ=255)                                                           aTGTTCATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCt        <  1:427555/81‑1 (MQ=255)                                                                cATCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCAc   >  2:427589/1‑81 (MQ=255)                                                                 aTCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACt  <  1:427557/81‑1 (MQ=255)                                                                 aTCTGCATTCCAGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACt  <  2:427558/81‑1 (MQ=255)                                                                            |                                                                      CTGGAAGATTCCCCGCAGTTACAGCAGGTGTTACCCGCTAACTGGTGGCAGGAAAACAATGTTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACT  >  NC_000913/2672203‑2672347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑