Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,182	C→T	T143T (ACC→ACT)	ung →	uracil‑DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,182	0	C	T	100.0%	68.4 / NA	21	T143T (ACC→ACT)	ung	uracil‑DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/8);  total (13/8)

GGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCG  >  NC_000913/2717104‑2717251                                                                               |                                                                      gggCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACttt                                                                     >  1:436147/1‑81 (MQ=255)          gCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATa                                                             >  2:436143/1‑80 (MQ=255)           cTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATa                                                             >  2:436145/1‑79 (MQ=255)                cAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGAt                                                      >  2:436156/1‑81 (MQ=255)                        tgtTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCt                                                <  1:436118/79‑1 (MQ=255)                        tgtTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTg                                               <  1:436119/80‑1 (MQ=255)                            gACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTaa                                          >  2:436142/1‑81 (MQ=255)                                 tACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAg                                      >  2:436152/1‑80 (MQ=255)                                      gcAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATcgc                                >  1:436163/1‑81 (MQ=255)                                       cAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATcgc                                >  1:436149/1‑80 (MQ=255)                                             aGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGc                          <  1:436121/80‑1 (MQ=255)                                             aGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGc                          <  1:436120/80‑1 (MQ=255)                                              ggCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCgt                        <  2:436123/81‑1 (MQ=255)                                              ggCGCATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCgt                        <  1:436122/81‑1 (MQ=255)                                                 gcATTCCCACGCCAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCgtggt                     >  1:436150/1‑81 (MQ=255)                                                  cATTCCCACTCCAGCCTCGGCTGGGATACTTTCTCCGATAGAGTGATCAGCCTGATTAACCAGCATCGCTAAGGCgtcgtg                    <  2:436124/81‑1 (MQ=255)                                                            ccAGCCTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTtgttgt          >  2:436171/1‑81 (MQ=255)                                                                ccTCGGCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTggg       >  2:436155/1‑80 (MQ=255)                                                                    ggCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGAt    <  2:436125/79‑1 (MQ=255)                                                                    ggCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCg  >  1:436158/1‑81 (MQ=255)                                                                    ggCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCg  >  1:436146/1‑81 (MQ=255)                                                                     gCTGGGAAACTTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCg  >  1:436153/1‑80 (MQ=255)                                                                               |                                                                      GGGCGTTCTGCTACTCAATACTGTGTTGACGGTACGCGCAGGTCAGGCGCATTCCCACGCCAGCCTCGGCTGGGAAACCTTCACCGATAAAGTGATCAGCCTGATTAACCAGCATCGCGAAGGCGTGGTGTTTTTGTTGTGGGGATCG  >  NC_000913/2717104‑2717251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑