Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,817	C→T	A238T (GCG→ACG)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,817	0	C	T	100.0%	105.4 / NA	30	A238T (GCG→ACG)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/13);  total (17/13)

CATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGC  >  NC_000913/2717739‑2717891                                                                               |                                                                           catcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGtcc                                                                          >  1:436286/1‑81 (MQ=255)     cAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGa                                                                      >  1:436293/1‑81 (MQ=255)            ttAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCcagc                                                               <  2:436255/81‑1 (MQ=255)               aCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGt                                                            <  1:436256/81‑1 (MQ=255)                  aTGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGcc                                                         <  1:436257/81‑1 (MQ=255)                    gttgtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCg                                                        >  2:436289/1‑80 (MQ=255)                         cGCCAGTAATCTGCTGAACGTGCTCGGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCt                                                  <  2:436258/81‑1 (MQ=255)                          gCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCt                                                  <  1:436259/80‑1 (MQ=255)                              gTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGcac                                              >  1:436298/1‑80 (MQ=255)                                    ggCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGac                                        <  1:436261/80‑1 (MQ=255)                                    ggCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGac                                        <  2:436260/80‑1 (MQ=255)                                        gAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                   >  1:436294/1‑81 (MQ=255)                                        gAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                   >  1:436301/1‑81 (MQ=255)                                         aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCttt                                  >  1:436307/1‑81 (MQ=255)                                            gTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCAc                               >  1:436302/1‑81 (MQ=255)                                            gTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCAc                               >  2:436297/1‑81 (MQ=255)                                              gCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGc                             >  1:436292/1‑81 (MQ=255)                                                 ctGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCg                           >  2:436295/1‑80 (MQ=255)                                                  tGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCg                           >  1:436303/1‑79 (MQ=255)                                                  tGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCg                           <  2:436262/79‑1 (MQ=255)                                                    cgccgcCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGa                          <  1:436263/78‑1 (MQ=255)                                                        gccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTCCACGCCGAAGTgcgc                   <  2:436264/81‑1 (MQ=255)                                                          cTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                  >  1:436311/1‑80 (MQ=255)                                                                cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTg            <  1:436265/80‑1 (MQ=255)                                                                 ggTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcg          >  2:436296/1‑81 (MQ=255)                                                                  gTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcg          >  2:436308/1‑80 (MQ=255)                                                                  gTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcg          >  2:436309/1‑80 (MQ=255)                                                                    aCGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcat       >  2:436304/1‑81 (MQ=255)                                                                       gATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatcat    <  2:436267/81‑1 (MQ=255)                                                                        aTAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATg   <  2:436268/81‑1 (MQ=255)                                                                         tAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGc  >  1:436310/1‑81 (MQ=255)                                                                               |                                                                           CATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGC  >  NC_000913/2717739‑2717891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑