Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,720,128	C→T	A59V (GCC→GTC)	patZ →	peptidyl‑lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,720,128	0	C	T	100.0%	90.6 / NA	26	A59V (GCC→GTC)	patZ	peptidyl‑lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

CCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTC  >  NC_000913/2720054‑2720205                                                                           |                                                                              ccTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTtggtctggcc                                                                         <  1:436783/81‑1 (MQ=255)    gctgGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGa                                                                      >  2:436829/1‑81 (MQ=255)     ctgGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCgg                                                                       <  1:436784/79‑1 (MQ=255)          gggAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGc                                                                >  2:436822/1‑81 (MQ=255)               gCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCt                                                           >  1:436839/1‑81 (MQ=255)               gCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCt                                                           >  2:436827/1‑81 (MQ=255)                cTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCtt                                                          <  1:436785/81‑1 (MQ=255)                   tAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGcc                                                       >  1:436824/1‑81 (MQ=255)                    aaCGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGcc                                                       <  1:436786/80‑1 (MQ=255)                     aCGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCt                                                     <  1:436787/81‑1 (MQ=255)                       ggACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCtt                                                    >  1:436818/1‑80 (MQ=255)                             gTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACAccc                                             >  2:436838/1‑81 (MQ=255)                             gTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACAccc                                             >  1:436832/1‑81 (MQ=255)                              tACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCg                                            <  2:436788/81‑1 (MQ=255)                              tACTCCCGGTGACGCCAGCCTGGAAAGCGGTATTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCg                                            <  2:436789/81‑1 (MQ=255)                                     ggTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGc                                     >  2:436836/1‑81 (MQ=255)                                      gTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCg                                    <  1:436790/81‑1 (MQ=255)                                       tGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCgg                                   >  2:436846/1‑81 (MQ=255)                                       tGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCgg                                   <  2:436791/81‑1 (MQ=255)                                        gACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGt                                  >  2:436849/1‑81 (MQ=255)                                        gACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGt                                  >  1:436823/1‑81 (MQ=255)                                          cGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGttt                                <  1:436792/81‑1 (MQ=255)                                                    gAAAGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTAcc                        <  2:436793/79‑1 (MQ=255)                                                     aaaGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAAt                     >  2:436842/1‑81 (MQ=255)                                                      aaGCGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATg                    >  1:436833/1‑81 (MQ=255)                                                         cGGTGTTGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCa                 <  1:436794/81‑1 (MQ=255)                                                              ttGGGTGTGTTGGTCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGt            <  2:436795/81‑1 (MQ=255)                                                                        tggtctggccGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGctc  >  1:436841/1‑81 (MQ=255)                                                                           |                                                                              CCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTC  >  NC_000913/2720054‑2720205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑