Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,733,607	A→G	R189R (CGT→CGC)	clpB ←	ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,733,607	0	A	G	100.0%	142.6 / NA	41	R189R (CGT→CGC)	clpB	ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/21);  total (20/21)

ATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTACGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGTCGA  >  NC_000913/2733530‑2733677                                                                              |                                                                       aTGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCgg                                                                     >  2:439718/1‑81 (MQ=255)    gCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTgcggcg                                                                   <  1:439667/80‑1 (MQ=255)          ttACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATttc                                                             <  1:439668/80‑1 (MQ=255)           tACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATttctt                                                           >  2:439714/1‑81 (MQ=255)           tACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATttctt                                                           >  2:439710/1‑81 (MQ=255)           tACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATttctt                                                           >  2:439700/1‑81 (MQ=255)           tACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATttctt                                                           >  2:439699/1‑81 (MQ=255)                  gCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCAc                                                     >  2:439704/1‑80 (MQ=255)                       gTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCaa                                               <  1:439669/81‑1 (MQ=255)                       gTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCaa                                               <  1:439670/81‑1 (MQ=255)                         tCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAAt                                              >  1:439706/1‑80 (MQ=255)                           aCCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCa                                            <  2:439671/80‑1 (MQ=255)                              aaTCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCgg                                        <  1:439672/81‑1 (MQ=255)                              aaTCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCg                                         >  2:439724/1‑80 (MQ=255)                                tCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGAt                                      >  1:439720/1‑81 (MQ=255)                                  aGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATc                                     <  2:439673/80‑1 (MQ=255)                                    tACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAg                                  <  2:439674/81‑1 (MQ=255)                                      ccGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGtt                                <  2:439675/81‑1 (MQ=255)                                      ccGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGtt                                <  2:439676/81‑1 (MQ=255)                                       cGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGttt                               >  1:439712/1‑81 (MQ=255)                                                tttAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTc                      >  1:439715/1‑81 (MQ=255)                                                 ttAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTc                      <  1:439677/80‑1 (MQ=255)                                                  tAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCTCCGGATCGAGTTTGCCCTGTTCg                     <  1:439678/80‑1 (MQ=255)                                                  tAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCgg                    >  2:439717/1‑81 (MQ=255)                                                   aGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGc                   >  2:439711/1‑81 (MQ=255)                                                     tACGACGTTGCAGCACCTGAATGGTGCGGCGGATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTc                 <  2:439679/81‑1 (MQ=255)                                                     tACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTc                 <  1:439680/81‑1 (MQ=255)                                                      acgacgTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCg                >  1:439727/1‑81 (MQ=255)                                                          cgTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCg            <  1:439681/81‑1 (MQ=255)                                                           gTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCgg           >  2:439716/1‑81 (MQ=255)                                                              gcagcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGt          >  1:439703/1‑79 (MQ=255)                                                              gcagcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTa         <  2:439682/80‑1 (MQ=255)                                                              gcagcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTa         <  1:439683/80‑1 (MQ=255)                                                               cagcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAg       <  2:439685/81‑1 (MQ=255)                                                               cagcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAg       <  1:439684/81‑1 (MQ=255)                                                                agcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAgg      >  1:439713/1‑81 (MQ=255)                                                                agcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAgg      <  1:439686/81‑1 (MQ=255)                                                                 gcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGt     <  2:439687/81‑1 (MQ=255)                                                                  caCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCATCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGTc    >  2:439719/1‑81 (MQ=255)                                                                  caCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGTc    >  2:439707/1‑81 (MQ=255)                                                                   aCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCCCGTTCGGTAAGGTc    >  1:439702/1‑80 (MQ=255)                                                                    ccTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGTCGa  <  1:439688/81‑1 (MQ=255)                                                                              |                                                                       ATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTACGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGTCGA  >  NC_000913/2733530‑2733677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑