Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,738,716	T→C	I324I (ATT→ATC)	pheA →	fused chorismate mutase/prephenate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,738,716	0	T	C	100.0%	71.5 / NA	21	I324I (ATT→ATC)	pheA	fused chorismate mutase/prephenate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

ACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCA  >  NC_000913/2738639‑2738789                                                                              |                                                                          aCGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCAt                                                                         >  1:440641/1‑80 (MQ=255)             gCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGaa                                                            >  1:440656/1‑81 (MQ=255)                 ccGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCAc                                                        <  1:440607/81‑1 (MQ=255)                 ccGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCAc                                                        <  2:440606/81‑1 (MQ=255)                     gCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACTATCTGATCATGACCCGTCTGGAATCACGccc                                                    >  2:440652/1‑81 (MQ=255)                     gCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGccc                                                    <  1:440609/81‑1 (MQ=255)                     gCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGccc                                                    <  2:440608/81‑1 (MQ=255)                           aGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCa                                              >  1:440649/1‑81 (MQ=255)                             ccGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACg                                            <  2:440610/81‑1 (MQ=255)                              cGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACgg                                           >  1:440645/1‑81 (MQ=255)                                    gcTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATcc                                     <  1:440611/81‑1 (MQ=255)                                    gcTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATc                                      >  1:440633/1‑80 (MQ=255)                                     cTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCa                                    >  1:440647/1‑81 (MQ=255)                                       ggTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATg                                  >  2:440651/1‑81 (MQ=255)                                                gTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGAt                         >  2:440648/1‑81 (MQ=255)                                                   gCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGt                       <  2:440612/80‑1 (MQ=255)                                                     tGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTc                     >  1:440653/1‑80 (MQ=255)                                                       gTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTa                   <  1:440613/80‑1 (MQ=255)                                                                   cacaATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAg      <  1:440614/81‑1 (MQ=255)                                                                      aaTCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGcc   <  1:440615/81‑1 (MQ=255)                                                                       aTCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa  <  1:440616/81‑1 (MQ=255)                                                                              |                                                                          ACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCA  >  NC_000913/2738639‑2738789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑