Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,755,418	C→A	intergenic (+40/‑175)	smpB → / → ssrA	SsrA‑binding protein/tmRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,755,418	0	C	A	100.0%	105.5 / NA	30	intergenic (+40/‑175)	smpB/ssrA	SsrA‑binding protein/tmRNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/19);  total (11/19)

ATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTCCCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCGA  >  NC_000913/2755344‑2755495                                                                           |                                                                              aTAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCtctc                                                                         <  2:443823/81‑1 (MQ=255)     aGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCcggc                                                                     <  1:443824/81‑1 (MQ=255)     aGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCcggc                                                                     <  2:443825/81‑1 (MQ=255)        aCGTATCATGAAAAACGCCCACCGTTAAACCAGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCggcggc                                                                  <  2:443826/81‑1 (MQ=255)            aTCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGcgcg                                                               >  2:443853/1‑80 (MQ=255)            aTCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGcgcg                                                               >  1:443863/1‑80 (MQ=255)             tCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGa                                                              <  2:443827/80‑1 (MQ=255)               aTGAAAAACGCCCACCGTTAAACCCGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATg                                                           <  1:443828/81‑1 (MQ=255)                     aaCGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATc                                                     <  1:443829/81‑1 (MQ=255)                      aCGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCt                                                    >  2:443859/1‑81 (MQ=255)                        gCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTa                                                  >  1:443878/1‑81 (MQ=255)                          ccACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTAtt                                                >  1:443865/1‑81 (MQ=255)                          ccACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTAtt                                                <  2:443830/81‑1 (MQ=255)                           cACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTg                                               <  2:443831/81‑1 (MQ=255)                             ccGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGc                                             >  2:443864/1‑81 (MQ=255)                              cGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCt                                            <  1:443832/81‑1 (MQ=255)                                   aaCCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATcac                                       <  1:443833/81‑1 (MQ=255)                                   aaCCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATca                                        >  1:443861/1‑80 (MQ=255)                                     ccTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACAt                                     <  2:443834/81‑1 (MQ=255)                                        gCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATcc                                   >  2:443879/1‑80 (MQ=255)                                         cACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATcc                                   <  2:443835/79‑1 (MQ=255)                                                attattGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAAt                          <  2:443836/81‑1 (MQ=255)                                                 ttattGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATg                         <  1:443837/81‑1 (MQ=255)                                                   attGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGtt                       >  1:443868/1‑81 (MQ=255)                                                           gTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATccc               <  2:443838/81‑1 (MQ=255)                                                              ccTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCAtt            >  2:443875/1‑81 (MQ=255)                                                                  aCCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGc          <  1:443839/79‑1 (MQ=255)                                                                     gcgcCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAAt     <  1:443840/81‑1 (MQ=255)                                                                       gcCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATc    >  2:443874/1‑80 (MQ=255)                                                                       gcCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCg   <  1:443841/81‑1 (MQ=255)                                                                        cctacctcTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCGa  <  2:443842/81‑1 (MQ=255)                                                                           |                                                                              ATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTCCCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCGA  >  NC_000913/2755344‑2755495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑