Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,579	T→C	I70V (ATC→GTC)	kbp ←	K(+) binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,579	0	T	C	100.0%	119.0 / NA	34	I70V (ATC→GTC)	kbp	K(+) binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

GGCAGTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGATTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTT  >  NC_000913/2796502‑2796654                                                                              |                                                                            ggcAGTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGActtt                                                                          >  2:450766/1‑81 (MQ=255)  gcAGTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTtc                                                                         >  1:450754/1‑81 (MQ=255)    aGTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTtctc                                                                       <  1:450717/81‑1 (MQ=255)        gCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTc                                                                   <  2:450718/81‑1 (MQ=255)          tGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGc                                                                 >  2:450757/1‑81 (MQ=255)              gtCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGcctcc                                                             >  1:450738/1‑81 (MQ=255)                cGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGcctcct                                                            >  2:450770/1‑80 (MQ=255)                cGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGcctcct                                                            >  2:450755/1‑80 (MQ=255)                   cGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACt                                                        <  2:450719/81‑1 (MQ=255)                    gTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTc                                                       <  1:450720/81‑1 (MQ=255)                      tttCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAg                                                     >  2:450761/1‑81 (MQ=255)                       ttCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAg                                                     <  2:450722/80‑1 (MQ=255)                       ttCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAg                                                     <  2:450721/80‑1 (MQ=255)                          aCCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGcc                                                  <  2:450723/80‑1 (MQ=255)                              gATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCAc                                             >  1:450765/1‑81 (MQ=255)                                 catcGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAg                                          >  1:450767/1‑81 (MQ=255)                                  atcGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAg                                          <  2:450724/80‑1 (MQ=255)                                      acacTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGAcc                                     <  1:450725/81‑1 (MQ=255)                                         cTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTc                                  <  2:450727/81‑1 (MQ=255)                                         cTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTc                                  <  2:450728/81‑1 (MQ=255)                                         cTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTc                                  <  1:450726/81‑1 (MQ=255)                                           ggCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGc                                >  2:450780/1‑81 (MQ=255)                                            gCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCt                               <  1:450729/81‑1 (MQ=255)                                              aaTACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCttt                             >  2:450764/1‑81 (MQ=255)                                                      aaaTATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCgg                     <  2:450730/81‑1 (MQ=255)                                                       aaTATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGTCCGTCGCTTTGCCGTCGGc                    <  1:450731/81‑1 (MQ=255)                                                          atTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAAt                 >  1:450771/1‑81 (MQ=255)                                                            tCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAAttt               >  2:450789/1‑81 (MQ=255)                                                               ccACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGaa            >  1:450777/1‑81 (MQ=255)                                                                cACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGaa            >  2:450784/1‑80 (MQ=255)                                                                   cGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATAtt        <  1:450732/81‑1 (MQ=255)                                                                   cGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATAtt        <  1:450733/81‑1 (MQ=255)                                                                    gCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTc       <  2:450734/81‑1 (MQ=255)                                                                         aaGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACttt  <  2:450735/81‑1 (MQ=255)                                                                         aaGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACtt   >  2:450781/1‑80 (MQ=255)                                                                              |                                                                            GGCAGTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGATTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTTT  >  NC_000913/2796502‑2796654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑