Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,126	T→C	F171L (TTC→CTC)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,126	0	T	C	100.0%	95.4 / NA	28	F171L (TTC→CTC)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/15);  total (13/15)

GGTGATTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCAC  >  NC_000913/2813053‑2813202                                                                          |                                                                             ggTGATTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTtcat                                                                       >  2:453894/1‑81 (MQ=255)  gTGATTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTtcatc                                                                      <  1:453846/81‑1 (MQ=255)  gTGATTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTtcatc                                                                      <  2:453847/81‑1 (MQ=255)     aTTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCaa                                                                    <  2:453848/80‑1 (MQ=255)        gTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGt                                                                 <  2:453849/80‑1 (MQ=255)         tCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGc                                                               <  1:453850/81‑1 (MQ=255)               cAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATtg                                                         >  1:453886/1‑81 (MQ=255)                    tGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGAAACTCTTCATCAACGTGCCGATtggcgtg                                                    >  1:453906/1‑81 (MQ=255)                            gATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCggtggt                                            >  2:453897/1‑81 (MQ=255)                            gATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCggtggt                                            <  2:453851/81‑1 (MQ=255)                              tCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGgtgt                                          >  2:453888/1‑81 (MQ=255)                                cTCGGCGGTTATATCAGCGATAATTAGCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTTGTGtt                                         >  1:453920/1‑80 (MQ=255)                                cTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGtt                                         >  1:453890/1‑80 (MQ=255)                                   ggcggTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTtgat                                      >  2:453896/1‑80 (MQ=255)                                   ggcggTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTtgat                                      >  2:453893/1‑80 (MQ=255)                                    gcggTTATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTtgatga                                    <  2:453852/81‑1 (MQ=255)                                        ttATATCAGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACt                                <  1:453853/81‑1 (MQ=255)                                               aGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAAct                         >  2:453895/1‑81 (MQ=255)                                               aGCGATAATTACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGACGGTGGTGTTGATGACACTGCaatc                          <  2:453854/80‑3 (MQ=255)                                                       ttACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCgg                 <  1:453855/81‑1 (MQ=255)                                                        tACCACTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGa                >  1:453910/1‑81 (MQ=255)                                                            aCTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGt             >  1:453901/1‑80 (MQ=255)                                                            aCTGAGGCTGGATACTCGTCATCAACGTAACGATTAGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTg            <  2:453856/81‑1 (MQ=255)                                                             cTGGGGCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGa           <  1:453857/81‑1 (MQ=255)                                                                gggCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGaa          <  2:453858/79‑1 (MQ=255)                                                                 ggCTGGATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAAcc       >  1:453899/1‑81 (MQ=255)                                                                      gATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGc    <  1:453859/79‑1 (MQ=255)                                                                      gATACTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCAc  <  1:453860/81‑1 (MQ=255)                                                                          |                                                                             GGTGATTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCAC  >  NC_000913/2813053‑2813202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑