Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,821,522	G→A	P164S (CCA→TCA)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,522	0	G	A	100.0%	130.7 / NA	36	P164S (CCA→TCA)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/26);  total (10/26)

GTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGC  >  NC_000913/2821445‑2821597                                                                              |                                                                            gTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGacgc                                                                          >  2:455536/1‑81 (MQ=255)    aTCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCAc                                                                        <  1:455490/80‑1 (MQ=255)     tCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCt                                                                      <  2:455491/81‑1 (MQ=255)       gTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCttt                                                                    >  2:455533/1‑81 (MQ=255)       gTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCttt                                                                    >  2:455530/1‑81 (MQ=255)       gTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCttt                                                                    >  1:455537/1‑81 (MQ=255)            agaTTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTAt                                                               <  2:455492/81‑1 (MQ=255)              aTTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCg                                                             <  1:455493/81‑1 (MQ=255)                 tCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGcc                                                           <  1:455494/80‑1 (MQ=255)                       cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCg                                                     <  1:455497/80‑1 (MQ=255)                       cACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGa                                                    <  2:455496/81‑1 (MQ=255)                       cACGGGCCGCACGGACCAGTGTCACCCATCTACCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGa                                                    <  2:455495/81‑1 (MQ=255)                          gggCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGaat                                                  >  1:455535/1‑80 (MQ=255)                           ggCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGaataa                                                <  2:455498/81‑1 (MQ=255)                            gCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGaataa                                                >  2:455543/1‑80 (MQ=255)                            gCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGAACCTTTGTTATCGCCGATGCGaataat                                               <  1:455499/81‑1 (MQ=255)                             ccGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAataata                                              >  1:455547/1‑81 (MQ=255)                             ccGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAataata                                              >  2:455540/1‑81 (MQ=255)                                    gACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgc                                       <  1:455500/81‑1 (MQ=255)                                     aCCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcg                                      <  1:455501/81‑1 (MQ=255)                                      ccAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgc                                     <  2:455502/81‑1 (MQ=255)                                          tgtCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGa                                 <  1:455503/81‑1 (MQ=255)                                            tCACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGAt                                <  1:455504/80‑1 (MQ=255)                                             cACCCATCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATcc                              >  1:455545/1‑81 (MQ=255)                                                  aTCTGCCAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTAct                         <  2:455505/81‑1 (MQ=255)                                                       ccAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCttt                    <  2:455506/81‑1 (MQ=255)                                                       ccAGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCttt                    <  2:455507/81‑1 (MQ=255)                                                         aGAAGTTGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCttttt                  <  1:455508/81‑1 (MQ=255)                                                              ttGTCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAg             <  2:455509/81‑1 (MQ=255)                                                                 tCAGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGAtt          <  1:455510/81‑1 (MQ=255)                                                                   aGATGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGAttt         <  1:455511/80‑1 (MQ=255)                                                                     aTGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCg       >  1:455542/1‑80 (MQ=255)                                                                      tGCGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCg       >  2:455538/1‑79 (MQ=255)                                                                        cGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAgg   <  2:455513/81‑1 (MQ=255)                                                                        cGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAg    <  2:455512/80‑1 (MQ=255)                                                                        cGTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAg    <  2:455514/80‑1 (MQ=255)                                                                         gTATGACGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGc  <  1:455515/81‑1 (MQ=255)                                                                              |                                                                            GTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGC  >  NC_000913/2821445‑2821597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑