Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,852,276	T→C	A47A (GCT→GCC)	hypD →	Fe‑(CN)2CO cofactor assembly scaffold protein HypD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,852,276	0	T	C	100.0%	97.8 / NA	29	A47A (GCT→GCC)	hypD	Fe‑(CN)2CO cofactor assembly scaffold protein HypD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/13);  total (16/13)

GAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGC  >  NC_000913/2852199‑2852351                                                                              |                                                                            gAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATc                                                                          >  2:462060/1‑81 (MQ=255) gAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATc                                                                          >  2:462058/1‑81 (MQ=255)     gTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCttt                                                                       >  2:462063/1‑80 (MQ=255)       gCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTaaa                                                                    >  2:462066/1‑81 (MQ=255)                   tctTACACCGCCGAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCg                                                          <  1:462024/79‑1 (MQ=255)                          ccgccgAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTAc                                                 >  2:462077/1‑81 (MQ=255)                           cgccgAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACt                                                <  1:462025/81‑1 (MQ=255)                           cgccgAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACt                                                <  1:462026/81‑1 (MQ=255)                           cgccgAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACt                                                <  2:462027/81‑1 (MQ=255)                            gccgAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTg                                               >  2:462069/1‑81 (MQ=255)                            gccgAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTg                                               <  2:462028/81‑1 (MQ=255)                             ccgAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGc                                              >  2:462062/1‑81 (MQ=255)                               gAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCg                                            <  2:462029/81‑1 (MQ=255)                               gAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCg                                            <  2:462030/81‑1 (MQ=255)                                        ctGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGtt                                   >  2:462070/1‑81 (MQ=255)                                          gCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGtt                                   >  1:462054/1‑79 (MQ=255)                                            ggATTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGt                               <  1:462031/81‑1 (MQ=255)                                              aTTATGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGtt                              >  1:462055/1‑80 (MQ=255)                                                 aTGGAAGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTAt                           <  1:462032/80‑1 (MQ=255)                                                      aGTGTGTGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACgg                     <  2:462033/81‑1 (MQ=255)                                                       gtgtgtGGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGt                    >  1:462068/1‑81 (MQ=255)                                                         gtgtgGCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTcc                  >  2:462071/1‑81 (MQ=255)                                                              gCGGTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCgggg              <  1:462034/80‑1 (MQ=255)                                                                ggTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGc           >  1:462102/1‑81 (MQ=255)                                                                 gTCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGcc          >  2:462064/1‑81 (MQ=255)                                                                  tCATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGccc         <  1:462035/81‑1 (MQ=255)                                                                   cATACCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCg        >  2:462065/1‑81 (MQ=255)                                                                      aCCCACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGgtg     >  2:462087/1‑81 (MQ=255)                                                                         cACGCCATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGc  <  1:462036/81‑1 (MQ=255)                                                                              |                                                                            GAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGATTATGGAAGTGTGTGGCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGC  >  NC_000913/2852199‑2852351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑