Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	293,374	A→G	V182A (GTT→GCT)	yagL ←	CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	293,374	0	A	G	100.0%	89.7 / NA	26	V182A (GTT→GCT)	yagL	CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/10);  total (16/10)

GAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGAC  >  NC_000913/293297‑293448                                                                              |                                                                           gAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCAt                                                                         >  2:61855/1‑81 (MQ=255)    tAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTc                                                                      <  2:61810/81‑1 (MQ=255)      aaTCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGa                                                                    >  1:61839/1‑81 (MQ=255)      aaTCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGa                                                                    <  2:61811/81‑1 (MQ=255)       aTCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGat                                                                   <  2:61812/81‑1 (MQ=255)       aTCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGa                                                                    >  1:61849/1‑80 (MQ=255)          cACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATc                                                                >  2:61842/1‑81 (MQ=255)                  ttAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCttt                                                          >  2:61848/1‑79 (MQ=255)                    aaGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAg                                                       <  1:61813/80‑1 (MQ=255)                         ttGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGAtt                                                 >  1:61858/1‑81 (MQ=255)                         ttGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGAtt                                                 >  1:61854/1‑81 (MQ=255)                         ttGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGAtt                                                 >  1:61852/1‑81 (MQ=255)                                atCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGAtt                                          >  2:61853/1‑81 (MQ=255)                                   aCGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATtgtg                                       <  2:61814/81‑1 (MQ=255)                                     gTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGaa                                     >  2:61847/1‑81 (MQ=255)                                       cGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATg                                   >  2:61850/1‑81 (MQ=255)                                       cGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATg                                   >  2:61851/1‑81 (MQ=255)                                               gCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCg                            <  2:61815/80‑1 (MQ=255)                                                cTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTa                          >  1:61816/1‑81 (MQ=255)                                                    cctcctTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCt                      <  1:61817/81‑1 (MQ=255)                                                       cctTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGAc                   >  2:61856/1‑81 (MQ=255)                                                        ctTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACg                  <  1:61818/81‑1 (MQ=255)                                                             gTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCa              <  1:61819/80‑1 (MQ=255)                                                                cTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATc           <  2:61820/80‑1 (MQ=255)                                                                cTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATc           >  1:61860/1‑80 (MQ=255)                                                                         tCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGAc  >  2:61859/1‑80 (MQ=255)                                                                              |                                                                           GAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAAGAC  >  NC_000913/293297‑293448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑