Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,858,782	A→C	T564P (ACC→CCC)	mutS →	DNA mismatch repair protein MutS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,858,782	0	A	C	100.0%	88.5 / NA	26	T564P (ACC→CCC)	mutS	DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/13);  total (13/13)

CTGGAAGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAA  >  NC_000913/2858707‑2858859                                                                            |                                                                              cTGGAAGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCt                                                                           >  1:463533/1‑80 (MQ=255)     aaGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAAc                                                                       >  1:463536/1‑80 (MQ=255)      aGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTa                                                                     >  2:463556/1‑81 (MQ=255)             cAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGc                                                              <  1:463483/81‑1 (MQ=255)             cAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGc                                                              <  2:463484/81‑1 (MQ=255)              aaCAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGcc                                                             <  1:463485/81‑1 (MQ=255)                 agagCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGa                                                          >  2:463537/1‑81 (MQ=255)                     cgcgagcgcgCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCtt                                                      <  2:463486/81‑1 (MQ=255)                        gagcgcgCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCAt                                                   <  1:463487/81‑1 (MQ=255)                        gagcgcgCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCAt                                                   >  2:463534/1‑81 (MQ=255)                          gcgcgcTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTg                                                 <  2:463488/81‑1 (MQ=255)                            gcgcTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGAt                                               <  1:463489/81‑1 (MQ=255)                                tGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAAc                                           >  1:463540/1‑81 (MQ=255)                                 ggCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAAcc                                          >  1:463522/1‑81 (MQ=255)                                  gCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCg                                         >  1:463544/1‑81 (MQ=255)                                  gCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCg                                         <  2:463490/81‑1 (MQ=255)                                           gACGTGCTGGTTAACCTGGCGGGTCGGACCTATCCCCTGAACTACACCGGCCCGACCTTCATTGATAAACCGAGCATTc                                  <  2:463491/79‑1 (MQ=255)                                             cGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCAt                              <  2:463492/81‑1 (MQ=255)                                                 cTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTAcc                          <  2:463493/81‑1 (MQ=255)                                                 cTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTAcc                          <  2:463494/81‑1 (MQ=255)                                                    gTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCg                         >  2:463557/1‑79 (MQ=255)                                                    gTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCg                         >  1:463550/1‑79 (MQ=255)                                                      tAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAgg                     >  2:463535/1‑81 (MQ=255)                                                               ggAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATcc            >  1:463549/1‑81 (MQ=255)                                                                gAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCg           >  1:463553/1‑81 (MQ=255)                                                                 aaCGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCgg          <  2:463495/81‑1 (MQ=255)                                                                           taCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGaa  <  2:463496/79‑1 (MQ=255)                                                                            |                                                                              CTGGAAGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAA  >  NC_000913/2858707‑2858859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑