Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,865,111	T→C	I4T (ATT→ACT)	ygbN →	putative transporter YgbN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,111	0	T	C	100.0%	123.0 / NA	36	I4T (ATT→ACT)	ygbN	putative transporter YgbN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/10);  total (26/10)

GTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTCG  >  NC_000913/2865036‑2865188                                                                            |                                                                              gTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACAt                                                                          >  2:464779/1‑81 (MQ=255)  tACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACAtt                                                                         >  2:464782/1‑81 (MQ=255)  tACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACAtt                                                                         <  1:464754/81‑1 (MQ=255)    cccATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGt                                                                       <  2:464755/81‑1 (MQ=255)      cATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTa                                                                     >  1:464784/1‑81 (MQ=255)         aCATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATg                                                                   >  1:464798/1‑80 (MQ=255)          cATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGc                                                                  <  1:464756/80‑1 (MQ=255)           aTCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCAt                                                                >  2:464787/1‑81 (MQ=255)             ccccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTg                                                              >  2:464792/1‑81 (MQ=255)              cccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGc                                                             >  2:464806/1‑81 (MQ=255)               ccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCg                                                            >  1:464802/1‑81 (MQ=255)                   aTCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAg                                                        >  1:464812/1‑81 (MQ=255)                      tACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTg                                                     >  2:464790/1‑81 (MQ=255)                       aCGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCTCAACTACATTGTTATGCATTGCGTTAGCTgg                                                    >  2:464799/1‑81 (MQ=255)                        cGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGc                                                   <  1:464757/81‑1 (MQ=255)                              gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAAt                                              >  1:464788/1‑80 (MQ=255)                               ggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCa                                            >  2:464796/1‑81 (MQ=255)                                    gTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTg                                       >  1:464789/1‑81 (MQ=255)                                     ttAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGt                                      >  1:464810/1‑81 (MQ=255)                                       aaCTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTtg                                    >  2:464795/1‑81 (MQ=255)                                          tataACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTtgctg                                 >  2:464807/1‑81 (MQ=255)                                             aaCTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGctgctg                              >  1:464805/1‑81 (MQ=255)                                              aCTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTgg                             <  2:464758/81‑1 (MQ=255)                                              aCTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTgg                             >  2:464794/1‑81 (MQ=255)                                               cTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGt                            >  1:464801/1‑81 (MQ=255)                                                tttCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGt                            >  1:464800/1‑80 (MQ=255)                                                  tCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGtcat                         >  2:464793/1‑81 (MQ=255)                                                    aGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGtcatc                        <  1:464759/80‑1 (MQ=255)                                                      aCAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAg                     <  1:464760/81‑1 (MQ=255)                                                            tttCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAg               >  2:464797/1‑81 (MQ=255)                                                            tttACCAAGTCCACAACTACATAGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAg               <  1:464761/81‑1 (MQ=255)                                                                ccATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTAc           <  1:464762/81‑1 (MQ=255)                                                                       cacaACTACGTTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCAtt    >  2:464809/1‑81 (MQ=255)                                                                        acaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTc   >  1:464803/1‑81 (MQ=255)                                                                        acaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTCTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTc   >  1:464763/1‑81 (MQ=255)                                                                         caACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTCg  <  2:464764/81‑1 (MQ=255)                                                                            |                                                                              GTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTCG  >  NC_000913/2865036‑2865188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑