Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,889,110	T→C	K330K (AAA→AAG)	cysI ←	sulfite reductase, hemoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,889,110	0	T	C	100.0%	127.6 / NA	36	K330K (AAA→AAG)	cysI	sulfite reductase, hemoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/26);  total (10/26)

CCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTG  >  NC_000913/2889032‑2889185                                                                               |                                                                            ccAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAActt                                                                           >  2:469387/1‑81 (MQ=255)  cAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAActt                                                                           >  2:469379/1‑80 (MQ=255)   aGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGa                                                                         >  2:469399/1‑81 (MQ=255)     ttATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATc                                                                       <  1:469337/81‑1 (MQ=255)     ttATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATc                                                                       <  2:469336/81‑1 (MQ=255)       atcatcAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATccc                                                                     >  1:469397/1‑81 (MQ=255)       atcatcAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATccc                                                                     >  1:469381/1‑81 (MQ=255)       atcatcAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATcc                                                                      <  1:469338/80‑1 (MQ=255)        tcatcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATcccc                                                                    <  1:469341/81‑1 (MQ=255)        tcatcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATcccc                                                                    <  2:469340/81‑1 (MQ=255)        tcatcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATccc                                                                     <  1:469339/80‑1 (MQ=255)               tGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGAc                                                             <  1:469343/81‑1 (MQ=255)               tGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGAc                                                             <  2:469342/81‑1 (MQ=255)                      aaCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTcc                                                        <  1:469344/79‑1 (MQ=255)                        cccAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACtt                                                    <  2:469345/81‑1 (MQ=255)                        cccAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACtt                                                    <  1:469346/81‑1 (MQ=255)                         ccAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTc                                                   <  2:469347/81‑1 (MQ=255)                         ccAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTc                                                   >  1:469389/1‑81 (MQ=255)                                aTACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTa                                            <  1:469348/81‑1 (MQ=255)                                 tACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTaa                                           <  1:469349/81‑1 (MQ=255)                                    gATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACg                                        >  2:469393/1‑81 (MQ=255)                                    gATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACg                                        <  1:469350/81‑1 (MQ=255)                                     aTCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGt                                       <  2:469351/81‑1 (MQ=255)                                          cTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCaa                                  <  1:469352/81‑1 (MQ=255)                                          cTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCaa                                  >  1:469388/1‑81 (MQ=255)                                             gTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAccc                               <  1:469354/81‑1 (MQ=255)                                             gTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAccc                               >  1:469394/1‑81 (MQ=255)                                             gTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAc                                 <  2:469353/79‑1 (MQ=255)                                               ccGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAcccc                              >  1:469405/1‑80 (MQ=255)                                                cGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCa                            <  1:469355/81‑1 (MQ=255)                                                 ggTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAc                           >  2:469391/1‑81 (MQ=255)                                                       cTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTc                     <  2:469356/81‑1 (MQ=255)                                                          atatGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCa                   <  2:469357/80‑1 (MQ=255)                                                             tGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCg                <  2:469358/80‑1 (MQ=255)                                                                     tCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTgg       <  1:469359/81‑1 (MQ=255)                                                                       ggTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGt      <  2:469360/80‑1 (MQ=255)                                                                       ggTTCAAACTTAATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGtt     >  1:469395/1‑81 (MQ=255)                                                                           cAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTg  <  1:469361/80‑1 (MQ=255)                                                                               |                                                                            CCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTG  >  NC_000913/2889032‑2889185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑