Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,902,559	G→A	D222N (GAT→AAT)	ygcE →	putative sugar kinase YgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,902,559	0	G	A	100.0%	108.3 / NA	31	D222N (GAT→AAT)	ygcE	putative sugar kinase YgcE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/10);  total (21/10)

TCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTA  >  NC_000913/2902485‑2902636                                                                           |                                                                              tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGaaa                                                                         >  2:472105/1‑81 (MQ=255)   ccGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGCGGGCCGGATTACTGGATAATGAAAc                                                                        <  2:472061/80‑1 (MQ=255)     ggCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAAcggc                                                                     >  2:472100/1‑81 (MQ=255)     ggCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAAcggc                                                                     >  2:472113/1‑81 (MQ=255)      gCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAAcggcg                                                                    >  2:472122/1‑81 (MQ=255)      gCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAAcggcg                                                                    >  1:472115/1‑81 (MQ=255)               ccGGTTGTTTGTACCACCAGTGATAATCCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGGAACGGCGGTAATTtc                                                            <  2:472062/80‑1 (MQ=255)                cGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTtctc                                                          >  2:472103/1‑81 (MQ=255)                cGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTtctc                                                          >  2:472097/1‑81 (MQ=255)                cGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTtctc                                                          <  1:472063/81‑1 (MQ=255)                 ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTctct                                                         >  1:472099/1‑81 (MQ=255)                 ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTctct                                                         >  1:472112/1‑81 (MQ=255)                 ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTctct                                                         <  2:472064/81‑1 (MQ=255)                   ttgtttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTg                                                       <  1:472065/81‑1 (MQ=255)                          tACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTa                                                >  2:472108/1‑81 (MQ=255)                           accaccAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTa                                                >  1:472117/1‑80 (MQ=255)                           accaccAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTa                                                >  2:472107/1‑80 (MQ=255)                                caGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCg                                           >  1:472106/1‑80 (MQ=255)                                 aGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCa                                         <  1:472066/81‑1 (MQ=255)                                    gATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAtt                                       >  2:472110/1‑80 (MQ=255)                                        aaCCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatg                                   <  1:472067/80‑1 (MQ=255)                                          ccGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatgat                                 >  2:472109/1‑80 (MQ=255)                                              tAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACg                            <  2:472068/81‑1 (MQ=255)                                                gAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGc                          <  1:472069/81‑1 (MQ=255)                                                      ctGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCa                    >  2:472114/1‑81 (MQ=255)                                                       tGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGc                     <  2:472070/79‑1 (MQ=255)                                                        gggggCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACt                  >  2:472119/1‑81 (MQ=255)                                                          gggCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGc                >  2:472126/1‑81 (MQ=255)                                                           ggCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGcc               >  2:472120/1‑81 (MQ=255)                                                            gCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGcc               >  1:472118/1‑80 (MQ=255)                                                                      tGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAg    >  1:472111/1‑81 (MQ=255)                                                                        gataatGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTa  <  2:472071/81‑1 (MQ=255)                                                                           |                                                                              TCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTA  >  NC_000913/2902485‑2902636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑