Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	298,160	C→G	A189A (GCG→GCC)	paoD ←	molybdenum cofactor insertion chaperone for PaoABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	298,160	0	C	G	100.0%	136.4 / NA	39	A189A (GCG→GCC)	paoD	molybdenum cofactor insertion chaperone for PaoABC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (23/16);  total (23/16)

AGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCA  >  NC_000913/298082‑298234                                                                               |                                                                           aGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTgggc                                                                          >  2:62633/1‑81 (MQ=255) aGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTgggc                                                                          >  2:62607/1‑81 (MQ=255)  gcgcTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGcc                                                                         <  1:62559/81‑1 (MQ=255)    gctggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCt                                                                        >  2:62583/1‑80 (MQ=255)    gctggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCt                                                                        >  1:62617/1‑80 (MQ=255)            aGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAAc                                                               <  1:62560/81‑1 (MQ=255)              gCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGt                                                             >  1:62609/1‑81 (MQ=255)                 ggAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCg                                                          >  1:62605/1‑81 (MQ=255)                 ggAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCg                                                          >  2:62620/1‑81 (MQ=255)                 ggAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCg                                                          >  2:62627/1‑81 (MQ=255)                  gAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCg                                                          >  1:62616/1‑80 (MQ=255)                   aaaaaGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTa                                                        <  1:62562/81‑1 (MQ=255)                   aaaaaGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTa                                                        <  1:62561/81‑1 (MQ=255)                       aGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAat                                                     >  1:62628/1‑80 (MQ=255)                        gATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAatca                                                   >  1:62606/1‑81 (MQ=255)                        gATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAatca                                                   <  2:62563/81‑1 (MQ=255)                           cGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAatcat                                                  >  2:62618/1‑79 (MQ=255)                           cGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAatcat                                                  >  2:62626/1‑79 (MQ=255)                              aGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGcc                                             <  2:62564/81‑1 (MQ=255)                                   tGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGAc                                         <  2:62565/80‑1 (MQ=255)                                       tGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATg                                    >  1:62600/1‑81 (MQ=255)                                        gTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATgg                                   >  1:62625/1‑81 (MQ=255)                                         tCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGc                                  <  2:62566/81‑1 (MQ=255)                                          cATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGcc                                 >  2:62614/1‑81 (MQ=255)                                               ccTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAAc                            <  1:62567/81‑1 (MQ=255)                                                 tgtgGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAAccc                          >  1:62621/1‑81 (MQ=255)                                                  gtgGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAAcccc                         <  2:62568/81‑1 (MQ=255)                                                   tgGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAAccc                          <  1:62569/79‑1 (MQ=255)                                                    ggCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCAc                       >  2:62637/1‑81 (MQ=255)                                                    ggCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCAc                       >  2:62608/1‑81 (MQ=255)                                                      ctgctgCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCt                     >  2:62612/1‑81 (MQ=255)                                                      ctgctgCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCt                     >  2:62636/1‑81 (MQ=255)                                                      ctgctgCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCt                     <  1:62570/81‑1 (MQ=255)                                                        gctgcAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTc                    >  1:62632/1‑80 (MQ=255)                                                         ctgcAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCaa                  <  2:62571/81‑1 (MQ=255)                                                            cAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGc               <  2:62572/81‑1 (MQ=255)                                                             aaGACCCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGc               >  2:62629/1‑80 (MQ=255)                                                               gACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCAt            <  1:62573/81‑1 (MQ=255)                                                                    gcgGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCAtt           >  1:62640/1‑77 (MQ=255)                                                                       gTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTc    >  2:62622/1‑81 (MQ=255)                                                                          gCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCa  <  2:62574/80‑1 (MQ=255)                                                                               |                                                                           AGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTCCA  >  NC_000913/298082‑298234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑