Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,928,914	C→T	S229F (TCT→TTT)	sdaC →	serine:H(+) symporter SdaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,928,914	0	C	T	100.0%	107.8 / NA	30	S229F (TCT→TTT)	sdaC	serine:H(+) symporter SdaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/20);  total (10/20)

AACGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTCTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCATTCGCA  >  NC_000913/2928841‑2928990                                                                          |                                                                             aaCGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTtttctttc                                                                       <  1:477273/81‑1 (MQ=255)  aCGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCg                                                                      <  2:477274/81‑1 (MQ=255)    ggTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGcc                                                                    <  1:477275/81‑1 (MQ=255)                aCCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGt                                                        <  2:477276/81‑1 (MQ=255)                    tgtgGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTg                                                       >  1:477326/1‑78 (MQ=255)                      tggctggcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAaga                                                   <  1:477277/80‑1 (MQ=255)                      tggctggcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAag                                                    <  2:477279/79‑1 (MQ=255)                      tggctggcAATTCCGGTAATGGTGTTCTCATTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAag                                                    <  2:477278/79‑1 (MQ=255)                        gctggcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGt                                                 <  2:477281/80‑1 (MQ=255)                        gctggcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGt                                                 <  1:477280/80‑1 (MQ=255)                            gcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGc                                            <  1:477282/81‑1 (MQ=255)                              aaTTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGa                                          >  1:477330/1‑81 (MQ=255)                               aTTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGat                                         <  2:477283/81‑1 (MQ=255)                                ttCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGata                                        <  1:477284/81‑1 (MQ=255)                                 tCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGatat                                       >  1:477307/1‑81 (MQ=255)                                 tCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGatat                                       >  1:477334/1‑81 (MQ=255)                                  ccGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGatat                                       <  1:477285/80‑1 (MQ=255)                                  ccGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATg                                      >  1:477338/1‑81 (MQ=255)                                     gTAATGGTGTTCTCGTTCAAACACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGca                                   <  1:477286/81‑1 (MQ=255)                                          ggTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcagaa                                >  2:477331/1‑79 (MQ=255)                                           gtgtTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcagaacag                             <  2:477287/81‑1 (MQ=255)                                                   gTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTc                     >  2:477332/1‑81 (MQ=255)                                                     tCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCg                    >  2:477335/1‑80 (MQ=255)                                                       aaCCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAg                 <  1:477288/81‑1 (MQ=255)                                                          cACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATc              <  1:477289/81‑1 (MQ=255)                                                            ctctCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCt            <  1:477290/81‑1 (MQ=255)                                                               tcCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTg           <  2:477291/79‑1 (MQ=255)                                                                 cGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCa        <  2:477292/80‑1 (MQ=255)                                                                 cGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCAt       <  2:477293/81‑1 (MQ=255)                                                                  gATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCAtt      >  2:477343/1‑81 (MQ=255)                                                                      atcTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCATTCGca  >  2:477333/1‑81 (MQ=255)                                                                          |                                                                             AACGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTCTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCATTCGCA  >  NC_000913/2928841‑2928990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑