Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,945,771	C→T	A24A (GCG→GCA)	tcdA ←	tRNA threonylcarbamoyladenosine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,945,771	0	C	T	100.0%	76.0 / NA	22	A24A (GCG→GCA)	tcdA	tRNA threonylcarbamoyladenosine dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/15);  total (7/15)

CCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCA  >  NC_000913/2945695‑2945846                                                                             |                                                                            ccAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCtt                                                                         <  2:480611/81‑1 (MQ=255)              cgccCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAg                                                            <  1:480613/81‑1 (MQ=255)              cgccCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAg                                                            <  2:480612/81‑1 (MQ=255)                  cAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAcgc                                                        >  2:480658/1‑81 (MQ=255)                  cAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAcgc                                                        >  2:480648/1‑81 (MQ=255)                   aGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAcgcg                                                       >  2:480661/1‑81 (MQ=255)                          gACACCACCGATACCGACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGc                                                 >  2:480655/1‑80 (MQ=255)                                 ccGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCaaaa                                         <  1:480614/81‑1 (MQ=255)                                    aTACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGc                                      <  2:480615/81‑1 (MQ=255)                                                 aaaTATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCAt                         <  1:480616/81‑1 (MQ=255)                                                      tGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTa                    <  2:480617/81‑1 (MQ=255)                                                         gcgTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAAtt                 <  2:480618/81‑1 (MQ=255)                                                          cgTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTa                <  1:480619/81‑1 (MQ=255)                                                           gTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTAc               <  1:480620/81‑1 (MQ=255)                                                            tCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTAcc              <  2:480622/81‑1 (MQ=255)                                                            tCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTAcc              <  1:480621/81‑1 (MQ=255)                                                                cAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAg          <  1:480623/81‑1 (MQ=255)                                                                 aaaCAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGa         >  2:480665/1‑81 (MQ=255)                                                                   aCAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACa       >  1:480663/1‑81 (MQ=255)                                                                    cAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACAt      <  1:480625/81‑1 (MQ=255)                                                                    cAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACAt      <  2:480624/81‑1 (MQ=255)                                                                        tGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCa  >  1:480668/1‑81 (MQ=255)                                                                             |                                                                            CCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCA  >  NC_000913/2945695‑2945846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑