Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,948,577	G→C	A145G (GCC→GGC)	amiC ←	N‑acetylmuramoyl‑L‑alanine amidase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,948,577	0	G	C	100.0%	122.6 / NA	35	A145G (GCC→GGC)	amiC	N‑acetylmuramoyl‑L‑alanine amidase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/24);  total (11/24)

GGCACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACG  >  NC_000913/2948499‑2948651                                                                               |                                                                           ggcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGccc                                                                          >  1:481191/1‑81 (MQ=255)  gcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCg                                                                         <  2:481134/81‑1 (MQ=255)   cACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCgg                                                                        <  1:481135/81‑1 (MQ=255)    aCCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGa                                                                       >  2:481189/1‑81 (MQ=255)    aCCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGa                                                                       >  1:481179/1‑81 (MQ=255)     ccTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGAt                                                                      <  2:481136/81‑1 (MQ=255)      cTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATa                                                                     <  2:481137/81‑1 (MQ=255)         cTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATaga                                                                   <  1:481138/80‑1 (MQ=255)              cGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTcc                                                              <  2:481140/80‑1 (MQ=255)              cGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTcc                                                              <  2:481139/80‑1 (MQ=255)               gAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCa                                                             <  2:481141/80‑1 (MQ=255)                aGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATc                                                           <  1:481142/81‑1 (MQ=255)                 ggTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCa                                                          >  2:481192/1‑81 (MQ=255)                       cTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGAc                                                    >  2:481194/1‑81 (MQ=255)                          tgttgtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACg                                                   <  2:481144/79‑1 (MQ=255)                          tgttgtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGc                                                  <  2:481143/80‑1 (MQ=255)                              gtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCtt                                              <  1:481145/80‑1 (MQ=255)                              gtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCtt                                              >  2:481190/1‑80 (MQ=255)                               tAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCttt                                             <  2:481146/80‑1 (MQ=255)                                       cagcagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCg                                      <  2:481147/79‑1 (MQ=255)                                       cagcagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGc                                    >  1:481203/1‑81 (MQ=255)                                           agcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGAcc                                <  1:481148/81‑1 (MQ=255)                                           agcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGAcc                                >  1:481193/1‑81 (MQ=255)                                                cagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgcgc                           <  2:481149/81‑1 (MQ=255)                                                    agcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAgg                       <  1:481150/81‑1 (MQ=255)                                                        ggTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCa                    >  2:481188/1‑80 (MQ=255)                                                         gTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCaaa                  >  1:481186/1‑81 (MQ=255)                                                            cTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAg               <  1:481151/81‑1 (MQ=255)                                                            cTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAg               >  1:481187/1‑81 (MQ=255)                                                                 tatCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCg          <  1:481152/81‑1 (MQ=255)                                                                  atCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCgg         >  2:481199/1‑81 (MQ=255)                                                                   tCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGt        <  2:481154/81‑1 (MQ=255)                                                                   tCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGt        <  2:481153/81‑1 (MQ=255)                                                                        tgCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGtttt     <  2:481155/79‑1 (MQ=255)                                                                        tgCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGtttt     <  2:481156/79‑1 (MQ=255)                                                                          cATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACg  <  2:481157/80‑1 (MQ=255)                                                                               |                                                                           GGCACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACG  >  NC_000913/2948499‑2948651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑