Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,960,513	T→C	D639G (GAT→GGT)	recC ←	exodeoxyribonuclease V subunit RecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,960,513	0	T	C	100.0%	158.6 / NA	45	D639G (GAT→GGT)	recC	exodeoxyribonuclease V subunit RecC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/23);  total (22/23)

ATCAGAGTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCATCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGCCATTGT  >  NC_000913/2960435‑2960587                                                                               |                                                                           aTCAGAGTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTtcacca                                                                          <  1:483701/81‑1 (MQ=255)   cAGAGTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACg                                                                        >  2:483768/1‑81 (MQ=255)    agagTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACg                                                                        <  2:483702/80‑1 (MQ=255)     gagTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGc                                                                       >  1:483760/1‑80 (MQ=255)     gagTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCa                                                                      <  1:483703/81‑1 (MQ=255)       gTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCaa                                                                     >  1:483750/1‑80 (MQ=255)            aaTGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGa                                                               >  1:483744/1‑81 (MQ=255)             aTGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGa                                                               >  1:483764/1‑80 (MQ=255)             aTGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGAc                                                              <  2:483704/81‑1 (MQ=255)                ttAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGc                                                           <  1:483705/81‑1 (MQ=255)                 tAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCg                                                          >  2:483763/1‑81 (MQ=255)                      ggTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCAcc                                                     >  2:483766/1‑81 (MQ=255)                      ggTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCAcc                                                     >  1:483757/1‑81 (MQ=255)                          cGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGt                                                 >  1:483743/1‑81 (MQ=255)                           ggCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTc                                                <  1:483706/81‑1 (MQ=255)                           ggCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTc                                                <  1:483707/81‑1 (MQ=255)                            gCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCg                                               >  2:483765/1‑81 (MQ=255)                              gagaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGcc                                             <  2:483709/81‑1 (MQ=255)                              gagaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGcc                                             <  1:483708/81‑1 (MQ=255)                              gagaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGc                                              >  2:483771/1‑80 (MQ=255)                              gagaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTGCACCACGCAATAGTGACAGCGGCACCGCGTCGcc                                             <  2:483710/81‑1 (MQ=255)                                gaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCa                                            >  2:483753/1‑80 (MQ=255)                                gaAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCAt                                           <  2:483711/81‑1 (MQ=255)                                 aaaaCGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATa                                          <  2:483712/81‑1 (MQ=255)                                  aaaCGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATAc                                         >  1:483756/1‑81 (MQ=255)                                    aCGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTg                                       <  1:483713/81‑1 (MQ=255)                                    aCGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTg                                       <  2:483714/81‑1 (MQ=255)                                      gctggctgATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTgcg                                     >  1:483740/1‑81 (MQ=255)                                            tgATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCt                                >  1:483755/1‑80 (MQ=255)                                             gATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTa                               >  2:483751/1‑80 (MQ=255)                                             gATACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTa                               >  2:483767/1‑80 (MQ=255)                                              aTACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTaaa                             <  2:483716/81‑1 (MQ=255)                                              aTACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTa                               <  1:483715/79‑1 (MQ=255)                                               tACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAAc                            >  1:483775/1‑81 (MQ=255)                                               tACGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAAc                            >  2:483762/1‑81 (MQ=255)                                                 cGTTCTTGATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCt                          >  2:483754/1‑81 (MQ=255)                                                        gATCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGc                     <  1:483717/79‑1 (MQ=255)                                                         aTCCAGACGCTGTGCCAGTTCACCACGCAATGGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGAt                  <  2:483718/81‑1 (MQ=255)                                                          tCCAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATa                 <  2:483719/81‑1 (MQ=255)                                                            cAGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAAt               <  1:483720/81‑1 (MQ=255)                                                             aGACGCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATc              >  2:483761/1‑81 (MQ=255)                                                                 gCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCt          <  2:483722/81‑1 (MQ=255)                                                                 gCTGTGCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCt          <  2:483721/81‑1 (MQ=255)                                                                   tgtgCCAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGc        >  2:483780/1‑81 (MQ=255)                                                                       ccAGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGCCAtt    <  1:483723/81‑1 (MQ=255)                                                                         aGTTCACCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGCCAttgt  <  1:483724/81‑1 (MQ=255)                                                                               |                                                                           ATCAGAGTACAAATGTTAACCGGTCCGGCGAGAAAACGCTGGCTGATACGTTCTTGATCCAGACGCTGTGCCAGTTCATCACGCAATAGTGACAGCGGCACCGCGTCGCCATACTGCGCACCTAAACCTTCGGCGATAATCGCCTGCCATTGT  >  NC_000913/2960435‑2960587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑