Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,986,420	C→T	H192Y (CAC→TAC)	yqeG →	putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,986,420	0	C	T	100.0%	78.0 / NA	22	H192Y (CAC→TAC)	yqeG	putative transporter YqeG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/11);  total (11/11)

TCCATTTACCTTGTCGGTAGTTGGCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGC  >  NC_000913/2986348‑2986490                                                                         |                                                                       tcCATTTACCTTGTCGGTAGTTGGCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGat                                                                 <  2:489031/80‑1 (MQ=255)   cATTTACCTTGTCGGTAGTTGGCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGatat                                                               <  2:489032/80‑1 (MQ=255)     tttACCTTGTCGGTAGTTGGCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGa                                                            >  1:489056/1‑81 (MQ=255)          cTTGTCGGTAGTTGGCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATc                                                        >  1:489053/1‑80 (MQ=255)               cGGTAGTTGGCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTcc                                                  >  2:489060/1‑81 (MQ=255)                ggTAGTTGGCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTccc                                                 >  1:489059/1‑81 (MQ=255)                        gCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATgg                                          <  2:489033/80‑1 (MQ=255)                        gCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGt                                         <  1:489035/81‑1 (MQ=255)                               gATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGcc                                  >  1:489066/1‑81 (MQ=255)                                    aTTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAg                             >  2:489057/1‑81 (MQ=255)                                    aTTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAg                             >  1:489062/1‑81 (MQ=255)                                     ttAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCtt                                <  2:489035/77‑1 (MQ=255)                                     ttAACAACCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGc                            <  1:489037/81‑1 (MQ=255)                                         caacCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATa                        <  2:489038/81‑1 (MQ=255)                                         caacCCAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATa                        <  1:489038/81‑1 (MQ=255)                                            cccAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGc                     <  1:489040/81‑1 (MQ=255)                                            cccAGGTAGAGTTCAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGACTTTAGCCATACGc                     >  1:489060/1‑81 (MQ=255)                                                         cAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa         <  1:489041/80‑1 (MQ=255)                                                         cAATCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTAc        >  2:489067/1‑81 (MQ=255)                                                          aaTCAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTAc        <  1:489042/80‑1 (MQ=255)                                                             cAGAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGttt    >  1:489064/1‑81 (MQ=255)                                                               gAATACCCTTTACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGc  >  2:489080/1‑81 (MQ=255)                                                                         |                                                                       TCCATTTACCTTGTCGGTAGTTGGCAACCTGATCTATTAACAACCCAGGTAGAGTTCAATCAGAATACCCTTCACCAGATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGC  >  NC_000913/2986348‑2986490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑