Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,754	C→T	R151Q (CGA→CAA)	ecpE ←	putative fimbrial chaperone EcpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,754	0	C	T	100.0%	119.8 / NA	34	R151Q (CGA→CAA)	ecpE	putative fimbrial chaperone EcpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/12);  total (22/12)

AAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATG  >  NC_000913/304678‑304830                                                                             |                                                                             aaaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtac                                                                          >  2:63948/1‑81 (MQ=255) aaaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtac                                                                          >  2:63939/1‑81 (MQ=255)  aaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtac                                                                          >  2:63938/1‑80 (MQ=255)       tgtgTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAg                                                                    <  1:63899/81‑1 (MQ=255)          gtTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAAt                                                                 >  1:63952/1‑81 (MQ=255)                aGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATc                                                           >  2:63944/1‑81 (MQ=255)                 gtgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATc                                                           >  1:63942/1‑80 (MQ=255)                  tgtTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCa                                                         >  1:63935/1‑81 (MQ=255)                     tacttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATca                                                      >  1:63936/1‑81 (MQ=255)                       cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                     >  2:63941/1‑80 (MQ=255)                          acCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcaccac                                                  >  1:63940/1‑80 (MQ=255)                             gTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCaccaccacc                                              <  1:63900/81‑1 (MQ=255)                             gTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCaccaccacc                                              >  2:63955/1‑81 (MQ=255)                             gTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCaccaccacc                                              >  1:63943/1‑81 (MQ=255)                              ttCATGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCg                                             <  2:63901/81‑1 (MQ=255)                               tCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCgg                                            >  1:63945/1‑81 (MQ=255)                                 cTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCt                                          <  1:63902/81‑1 (MQ=255)                                    tCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTc                                         <  2:63903/79‑1 (MQ=255)                                      aCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTg                                     <  1:63904/81‑1 (MQ=255)                                      aCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTg                                     >  1:63951/1‑81 (MQ=255)                                        cTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCt                                   >  1:63958/1‑81 (MQ=255)                                           gTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                                >  2:63910/1‑81 (MQ=255)                                            tCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                                <  2:63905/80‑1 (MQ=255)                                            tCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                                >  1:63954/1‑80 (MQ=255)                                            tCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                                >  1:63957/1‑80 (MQ=255)                                              gAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAc                             <  1:63906/81‑1 (MQ=255)                                                aGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgc                           >  2:63960/1‑81 (MQ=255)                                                         tAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGtt                   <  2:63907/80‑1 (MQ=255)                                                          aaaCTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTgg                 >  2:63947/1‑81 (MQ=255)                                                             cTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCt              <  2:63908/81‑1 (MQ=255)                                                             cTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCt              <  2:63909/81‑1 (MQ=255)                                                              tGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTg             >  2:63959/1‑81 (MQ=255)                                                               gAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGc            >  2:63950/1‑81 (MQ=255)                                                                         tGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATg  <  1:63910/81‑1 (MQ=255)                                                                             |                                                                             AAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATG  >  NC_000913/304678‑304830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑