Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,993,754	T→A	D39E (GAT→GAA)	ygeI →	protein YgeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,993,754	0	T	A	100.0%	43.1 / NA	13	D39E (GAT→GAA)	ygeI	protein YgeI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/1);  total (12/1)

CAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAG  >  NC_000913/2993678‑2993815                                                                             |                                                              cAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACaaa                                                           >  1:489927/1‑81 (MQ=255)   aaTATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATa                                                         >  1:489928/1‑81 (MQ=255)        aGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAg                                                     >  2:489928/1‑80 (MQ=255)        aGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAg                                                     >  2:489929/1‑80 (MQ=255)               gCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAGCATTta                                               >  1:489933/1‑79 (MQ=255)                 aaCGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAGCATTTATAGt                                           >  2:489944/1‑81 (MQ=255)                     ggTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAGCATTTATAGTTTCa                                       <  1:489914/81‑1 (MQ=255)                           gaAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAGCATTTATAGTTTCAACatg                                  >  2:489937/1‑80 (MQ=255)                                   aTCATTAGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAGCATTTATAGTTTCAACATGGATGGCGcc                         >  1:489940/1‑81 (MQ=255)                                         aGATAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAGCACTTATAGTTTCAACATGGATGGCGCCTTTTc                    >  2:489942/1‑80 (MQ=255)                                           aTAAACACATCAACACCAGTGCTACTGATACAGAACAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCaa                  >  1:489958/1‑80 (MQ=255)                                                        cacCAGTGCTACTGATACAGAACAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTAtt     >  1:489937/1‑80 (MQ=255)                                                           cAGTGCTACTGATACAGAACAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCag  >  2:489938/1‑80 (MQ=255)                                                                             |                                                              CAAATATAGCGAATGCAACGGGTGATGAAGTCGTATCATTAGATAAACACATCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAG  >  NC_000913/2993678‑2993815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑