Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,998,727	G→A	N34N (AAC→AAT)	ygeQ ←	protein YgeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,998,727	0	G	A	100.0%	86.4 / NA	25	N34N (AAC→AAT)	ygeQ	protein YgeQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/13);  total (12/13)

AGACGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGA  >  NC_000913/2998650‑2998802                                                                              |                                                                            agaCGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATAtt                                                                           >  2:490641/1‑80 (MQ=255) agaCGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTa                                                                          <  2:490613/81‑1 (MQ=255)  gaCGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTaa                                                                         <  2:490614/81‑1 (MQ=255)         aTTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGa                                                                  <  2:490615/81‑1 (MQ=255)                  cTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGt                                                         <  1:490617/81‑1 (MQ=255)                   ttATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGt                                                         >  2:490645/1‑80 (MQ=255)                     aTCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTc                                                        <  2:490617/79‑1 (MQ=255)                     aTCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAACTAAGAATATTAAATACAGATTCACTCGTCAc                                                      >  2:490639/1‑81 (MQ=255)                             ttAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTGccc                                              >  1:490641/1‑81 (MQ=255)                                         aaCTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCa                                 >  2:490644/1‑81 (MQ=255)                                          aCTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCAt                                <  1:490619/81‑1 (MQ=255)                                                aTCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGcc                           <  2:490619/80‑1 (MQ=255)                                                    gTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTCCCTCAc                       >  2:490649/1‑80 (MQ=255)                                                     ttGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCAc                       <  2:490620/79‑1 (MQ=255)                                                      tgctgcCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGa                     >  2:490642/1‑80 (MQ=255)                                                          gcCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAata                >  1:490644/1‑81 (MQ=255)                                                          gcCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAata                >  2:490647/1‑81 (MQ=255)                                                          gcCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAata                >  2:490656/1‑81 (MQ=255)                                                           cccccGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAatat               <  2:490621/81‑1 (MQ=255)                                                                   gAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCg        <  2:490623/80‑1 (MQ=255)                                                                   gAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCg        <  1:490623/80‑1 (MQ=255)                                                                       tAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGTATGTGCCGCACGAAAATATACCAGCGAg      >  2:490646/1‑78 (MQ=255)                                                                        aaGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCag   <  1:490625/80‑1 (MQ=255)                                                                        aaGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCag   >  2:490651/1‑80 (MQ=255)                                                                         aGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCaga  <  1:490626/80‑1 (MQ=255)                                                                              |                                                                            AGACGATAATTAGGATACTTATCTGGATTTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGA  >  NC_000913/2998650‑2998802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑