Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,999,715	G→A	T59T (ACC→ACT)	ygeR ←	LysM domain‑containing putative peptidase lipoprotein YgeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,999,715	0	G	A	100.0%	110.7 / NA	31	T59T (ACC→ACT)	ygeR	LysM domain‑containing putative peptidase lipoprotein YgeR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/19);  total (12/19)

ACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGT  >  NC_000913/2999637‑2999792                                                                               |                                                                              acccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGt                                                                             >  2:490863/1‑81 (MQ=255)      gTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCg                                                                        <  1:490812/81‑1 (MQ=255)          tAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCgtggt                                                                    >  2:490866/1‑81 (MQ=255)            gTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCgtggtg                                                                   <  2:490812/80‑1 (MQ=255)            gTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTgc                                                                  >  1:490856/1‑81 (MQ=255)             tttCTGACCAACTTCAATGGTGTAAGGGGGGAAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTgcg                                                                 <  2:490813/81‑1 (MQ=255)                        cTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGat                                                      <  1:490815/81‑1 (MQ=255)                           cAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGatat                                                    <  1:490817/80‑1 (MQ=255)                           cAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGatat                                                    <  2:490815/80‑1 (MQ=255)                            aaTGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGAtata                                                   <  2:490817/80‑1 (MQ=255)                            aaTGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAg                                                  >  1:490862/1‑81 (MQ=255)                             aTGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGc                                                 <  1:490819/81‑1 (MQ=255)                               ggTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCg                                                >  2:490859/1‑80 (MQ=255)                                gtgtAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTa                                              >  2:490862/1‑81 (MQ=255)                                  gtAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATc                                            >  2:490860/1‑81 (MQ=255)                                   tAAGGGGGGGAAATGCCGTTCAGTCGCGCCGGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATc                                            <  2:490819/80‑1 (MQ=255)                                              aaTGCCGTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc                                <  2:490820/81‑1 (MQ=255)                                                    gTTCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTa                          <  2:490821/81‑1 (MQ=255)                                                     ttCAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTa                          >  2:490868/1‑80 (MQ=255)                                                       cAGTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTaaa                        <  2:490822/80‑1 (MQ=255)                                                         gTCGCGCCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACgg                     <  2:490823/81‑1 (MQ=255)                                                              gcCAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCg                <  1:490825/81‑1 (MQ=255)                                                                cAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCgg               >  2:490864/1‑80 (MQ=255)                                                                cAGTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCgg               >  2:490874/1‑80 (MQ=255)                                                                  gTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa             >  2:490877/1‑80 (MQ=255)                                                                  gTTCTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAAt            <  1:490826/81‑1 (MQ=255)                                                                     cTTTTACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACg         >  2:490869/1‑81 (MQ=255)                                                                        ttACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACg         <  2:490827/78‑1 (MQ=255)                                                                        ttACGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACg         <  1:490827/78‑1 (MQ=255)                                                                          aCGCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTcc     <  2:490828/80‑1 (MQ=255)                                                                            gCTAGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGt  <  2:490829/81‑1 (MQ=255)                                                                               |                                                                              ACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGT  >  NC_000913/2999637‑2999792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑